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| Status |
Public on Mar 27, 2014 |
| Title |
Analysis of the DNA methylome and transcriptome in granulopoiesis reveal timed changes and dynamic enhancer methylation |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
The study investigated the changes in DNA-methylation and gene expression during granulopoiesis. To this end CMP, GMP, PMC and PMN cell populations were isolated by FACS from bone marrow from healthy donors and analysed by CAGE as well as expression and DNA microarrays.
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| Overall design |
Bisulphite converted DNA from biological triplicate samples of CMP, GMP, PMC and PMN cell poplulations were hybridised to the Illumina Infinium 450k Human Methylation Beadchip.
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| Contributor(s) |
Rönnerblad M, Olofsson T, Andersson R, Douagi I, Karimi M, Lehmann S, Hoof I, Sandelin A, Itoh M, Nago-Sato S, Kawaji H, Lassmann T, Carninici P, Hayashizaki Y, Forrest AR, Ekwall K, Arner E, Lennartsson A |
| Citation(s) |
24671952 |
| Submission date |
Sep 11, 2013 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Karl Ekwall |
| E-mail(s) |
karl.ekwall@ki.se
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| Phone |
+46 8 6089133
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| Organization name |
Karolinska Inst
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| Street address |
Alfred Nobels Alle 7
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| City |
Stockholm |
| ZIP/Postal code |
S-141 89 |
| Country |
Sweden |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA219099 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50797_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE50797_methylated_unmethylated_signal_intensities.txt.gz |
30.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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