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Series GSE50734 Query DataSets for GSE50734
Status Public on Nov 04, 2013
Title TEL-AML1 (ETV6-RUNX1) in B-cells and leukemia (part 5)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We identified directly and indirectly regulated target genes utilizing an inducible TEL-AML1 system derived from the murine pro B-cell line BA/F3 and a monoclonal antibody directed against TEL-AML1. By integration of promoter binding identified with ChIP-on-chip, gene expression and protein output through microarray technology and stable labelling of amino acids in cell culture (SILAC), we identified directly and indirectly regulated targets of the TEL-AML1 fusion protein.
Overall design Bound promoter regions of immunoprecipitated TEL-AML1 associated genes were first compared to input material and enrichment was calculated. The same was performed for empty vector control cell lines, also treated with the induction reagent mifepriston. Enriched promoter regions were then compared of the both sets. 2 independent replicates each (4 arrays) were performed.
Contributor(s) Linka Y, Ginzel S, Krüger M, Novosel A, Gombert M, Kremmer E, Harbott J, Thiele R, Borkhardt A, Landgraf P
Citation(s) 24121163, 26484077
Submission date Sep 10, 2013
Last update date Jun 07, 2019
Contact name Pablo Landgraf
Organization name Heinrich-Heine University Düsseldorf
Department Clinic of Pediatric Oncology, Hematology and Clinical Immunology
Street address Moorenstraße 5
City Düsseldorf
ZIP/Postal code 40225
Country Germany
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM1227397 bone_marrow_TA+_B-ALL_pat3
GSM1227398 bone_marrow_TA+_B-ALL_pat4
GSM1227399 bone_marrow_TA+_B-ALL_pat1
This SubSeries is part of SuperSeries:
GSE50736 TEL-AML1 (ETV6-RUNX1) in B-cells and leukemia
BioProject PRJNA218612

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50734_RAW.tar 78.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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