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Status |
Public on Dec 12, 2013 |
Title |
Coordinated genomic control of ciliogenesis and cell movement by Rfx2 |
Organism |
Xenopus laevis |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We have performed a systems-level analysis of the RFX/Daf-19 family transcription factor, Rfx2. Using a combination of high-throughput sequencing of Rfx2-regulated transcripts and chromosomal binding sites, we provide a comprehensive accounting of the target genes by which Rfx2 controls ciliogenesis and cilia beating in vertebrates.
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Overall design |
RNA-seq: two biological replicates for control and RFX2 knockdown by morpholino injection, ChIP-seq: RFX2-GFP pulldown with GFP antibody, GFP only expression used as control
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Contributor(s) |
Chung M, Kwon T, Gupta R, Baker JC, Marcotte EM, Wallingford JB |
Citation(s) |
24424412, 25419512 |
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Submission date |
Sep 04, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Taejoon Kwon |
E-mail(s) |
tkwon@unist.ac.kr
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Organization name |
Ulsan National Institute of Science and Technology
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Department |
Department of Biomedical Engineering
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Street address |
50 Unist-gil
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City |
Ulsan |
ZIP/Postal code |
44919 |
Country |
South Korea |
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Platforms (1) |
GPL17682 |
Illumina HiSeq 2000 (Xenopus laevis) |
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Samples (6)
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Relations |
BioProject |
PRJNA218018 |
SRA |
SRP029582 |