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Series GSE50591 Query DataSets for GSE50591
Status Public on Sep 05, 2013
Title Transcriptional Profiling Defines the Roles of ERK and p38 Kinases in Epidermal Keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Epidermal keratinocytes respond to extracellular influences by activating cytoplasmic signal transduction pathways that change the transcriptional profiles of affected cells. To define responses to two such pathways, p38 and ERK, we used SB203580 and PD98059 as specific inhibitors, and identified the regulated genes after 1, 4, 24 and 48 hrs, using Affymetrix’ Hu133Av2 microarrays. Additionally, we compared genes specifically regulated by p38 and ERKs with those regulated by JNK and by all three pathways simultaneously. We find that the p38 pathway induces the expression of extracellular matrix and proliferation-associated genes, while suppressing microtubule-associated genes; the ERK pathway induces the expression of nuclear envelope and mRNA splicing proteins, while suppressing steroid synthesis and mitochondrial energy production enzymes. Both pathways promote epidermal differentiation and induce feedback inactivation of MAPK signaling. c-FOS, SRY and N-Myc appear to be the principal targets of the p38 pathway, Elk-1 SAP1 and HLH2 of ERK, while FREAC-4, ARNT and USF are common to both. The results for the first time comprehensively define the genes regulated by the p38 and ERK pathways in epidermal keratinocytes and suggest a list of targets potentially useful in therapeutic interventions.
Overall design Human epidermal keratinocytes are grown in Keratinocyte Serum-Free Medium (Gibco) supplemented with 0.05 mg/ml bovine pituitary extract, 2.5 ng/ml epidermal growth factor, 0.09 mM CalCl2 and 1% penicillin/streptomycin (KGM). They are switched to Keratinocyte Serum Free-Media (Gibco) supplemented only with 1% penicillin/streptomycin (KBM) 24 h prior to commencing experiments. A set is left as controls, others treated with 5 uM JNK inhibitor SP600125, 15 uM p38 inhibitor SB203580, or 50 um ERK inhibitor PD98059. Timecourse of treated and parellel control samples over a 48 hr period was performed.
Contributor(s) Gazel A, Nijhawan RI, Walsh R, Blumenberg M
Citation(s) 18247374
Submission date Sep 04, 2013
Last update date Dec 06, 2018
Contact name Miroslav Blumenberg
Phone 212 263-5924
Organization name NYU School of Medicine
Department Dermatology
Lab Blumenberg
Street address 455 First Ave #874
City New York
State/province NY
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (16)
GSM1224304 Keratinocytes untreated 1h
GSM1224305 Keratinocytes untreated 4h
GSM1224306 Keratinocytes untreated 24h rep1
BioProject PRJNA218008

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Supplementary file Size Download File type/resource
GSE50591_RAW.tar 26.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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