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Series GSE50543 Query DataSets for GSE50543
Status Public on May 29, 2014
Title Trophoblast giant cell underrepresentation (CGH)
Organism Mus musculus
Experiment type Genome variation profiling by genome tiling array
Summary We characterized regions of underrepresentation that are specific to mouse polyploid trophoblast giant cells.
Overall design We performed array Comparative Genomics Hybridization (aCGH) to examine copy number variation (CNV) in mouse polyploid trophoblast giant cells (TGCs). We performed the following experiments in duplicates to examine CNV during various stages of in vivo and in vitro TGC development: e9.5 TGCs vs. embryonic controls, e11.5 TGCs vs. embryonic controls, e13.5 TGCs vs. embryonic controls, e16.5 TGCs vs. embryonic controls, as well as TGCs cultured 3, 5 and 7 days vs. 2N trophoblast stem cells. We also performed the following controls to show that underrepresentation is only found in polypoid trophoblast giant cells and not in either 2N placental cell types nor in other types of polyploid cells: 2N placenta disk vs. embryonic controls, 2N trophoblast stem cells vs. embryonic stem cells, and polyploid Megakaryocytes vs. embryonic controls. When possible, we performed arrays with the test and control samples of opposite sex (F-female, M-male), as an internal control for the array.
Contributor(s) Chuong EB, Hannibal RL, Baker JC
Citation(s) 24785991
Submission date Sep 03, 2013
Last update date Aug 28, 2014
Contact name Roberta L Hannibal
Organization name Stanford University
Department Genetics
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL10449 Agilent-027411 SurePrint G3 Mouse CGH Microarray 4x180K (Feature Number version)
Samples (20)
GSM1220962 Megakaryocytes vs. embryos (FvM)
GSM1220963 Megakaryocytes vs. embryos (MvF)
GSM1220964 e9.5 vs. embryos (FvM)
This SubSeries is part of SuperSeries:
GSE50585 Trophoblast giant cell underrepresentation
BioProject PRJNA217981

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50543_RAW.tar 374.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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