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Series GSE50429 Query DataSets for GSE50429
Status Public on Sep 01, 2013
Title Deep sequencing of exosomal and cellular miRNAs in MDA-MB-231 and MCF-10A cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary To identify the miRNAs that are differentially expressed and secreted between the MDA-MB-231 metastatic breast cancer cells and the MCF-10A non-cancerous human mammary epithelial cells, we profiled the cellular and exosomal small RNAs (between 17 and 52 nt) isolated from these two cell lines by Solexa deep sequencing. MiRNAs that are significantly different between the two cell lines are identified.
Overall design RNA was extracted from cultured MDA-MB-231 and MCF-10A cells or purified exosomes secreted by these cells, and subjected to library construction and Solexa deep sequencing.
Contributor(s) Wu X, Wang SE
Citation(s) 24735924
Submission date Aug 29, 2013
Last update date May 15, 2019
Contact name Xiwei Wu
Organization name Beckman Research Institute
Department Molecular and Cellular Biology
Lab Integrative Genomics Core
Street address 1500 E Duarte Rd
City Duarte
State/province CA
ZIP/Postal code 91010
Country USA
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (4)
GSM1219133 747_MDA-MB-231
GSM1219134 841_MDA-MB-231
GSM1219135 749_MCF-10A
BioProject PRJNA217805
SRA SRP029401

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50429_RAW.tar 30.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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