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Series GSE50423 Query DataSets for GSE50423
Status Public on Oct 02, 2014
Title Contextual fear conditioning in the mouse hippocampus
Organism Mus musculus
Experiment type Expression profiling by array
Summary A fundamental question in neuroscience is how memories are stored and retrieved in the brain. Many neurological, psychiatric and neurodevelopmental disorders are associated with cognitive deficits. Therefore characterizing the biological basis of these processes is critical for understanding normal and abnormal brain function. It is known that long-term memory formation requires transcription and translation as well as epigenetic processes that control gene expression. In this study we examined genome-wide gene expression changes during memory consolidation and after memory retrieval. We observe the largest changes in gene expression 30 minutes after memory acquisition and retrieval, and several novel genes were found to be affected by both. Interestingly, acquisition and retrieval of memory down-regulate different processes. Chromatin assembly is down-regulated after memory acquisition whereas RNA processing is down-regulated so after retrieval. Histone variant H2AB levels are reduced following acquisition, while splicing factor Rbfox1 and NMDA receptor-dependent microRNA miR-219 are down-regulated following retrieval. We also show that miR-219 down-regulation after retrieval is accompanied by up-regulation of its target protein CAMKII╬│. Our study highlights for the first time the differential involvement of epigenetic mechanisms that control gene expression, such as histone variants and post-transcriptional RNA processing, during memory acquisition and retrieval.
Overall design 72 total samples were analyzed, including animals trained in a contexual conditioning paradigm (FC) and controls. Tissue was collected at 30 minutes (FC30), 4 hours (FC4), 12 hours (FC12) and 24 hours after FC (FC24) as well as 30 minutes after testing for retrieval of the memory (RT30). Testing was performed at 24 hours after training over a 5-minute interval. Animals that were handled but not trained were dissected at the same time of day to control for variations due to circadian rhythms (CC30, CC4 andCC12). The protocol was repeated over the course of 2 weeks to obtain 9 animals (2 hippocampi) per group, so that 9 independent FC experiments were represented in each time point and all animals for each group were dissected at the exactly the same time of day.
Contributor(s) Peixoto L, Abel T
Citation(s) 26040834
Submission date Aug 28, 2013
Last update date Apr 18, 2017
Contact name Lucia Peixoto
Phone 5093686764
Organization name Washington State University, Spokane
Department College of Medicine
Street address PO Box 1495
City Spokane
State/province WA
ZIP/Postal code 99224
Country USA
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (72)
GSM1218964 Hippocampus_control_30minutes_rep1
GSM1218965 Hippocampus_control_30minutes_rep2
GSM1218966 Hippocampus_control_30minutes_rep3
BioProject PRJNA217484

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50423_RAW.tar 329.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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