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Series GSE49990 Query DataSets for GSE49990
Status Public on Oct 17, 2013
Title Dynamic analysis of gene expression and genome wide transcription factor binding during lineage-specification of multipotent progenitors [ER]
Organism Mus musculus
Experiment type Expression profiling by array
Summary FDCPmix cells were infected with Gata1 and Pu1 ER fusion proteins and microarrayed after induction to reveal genes regulated by each factor as part of a study investigating the differentiation of a multipotential cell-line to erthroid and myeloid fates.
We used the paradigmatic 'GATA-PU.1 axis’ to explore, at systems-level, dynamic relationships between transcription factor (TF) binding and global gene expression programs as multipotent cells differentiate. We combined global ChIPSeq of GATA1, GATA2 and PU.1 with expression profiling during differentiation to erythroid and neutrophil lineages. Our analysis reveals (i) differential complexity of sequence motifs bound by GATA1, GATA2 and PU.1; (ii) the scope and interplay of the GATA1 and GATA2 programs within, and during transitions between, different cell compartments, and the extent of their hard-wiring by DNA motifs; (iii) the potential to predict gene expression trajectories based on global associations between TF-binding data and target gene expression and (iv) how dynamic modeling of DNA-binding and gene expression data can be used to infer regulatory logic of TF circuitry. This 'rubric' exemplifies the utility of this cross-platform resource for deconvoluting the complexity of transcriptional programs controlling stem/progenitor cell fate in hematopoiesis.
Overall design FDCPmix cells were inected with lentivirus containing either Gata1 or Pu1 ER fusion protein, or a empty vector. Cells were sort on GFP and tamoxifen added after a period of growth. Expression arrays were performed at the beginning, and 24hr after induction of the ER fusion construct.
Contributor(s) Enver T, Soneji S
Citation(s) 24120743
Submission date Aug 19, 2013
Last update date Jan 12, 2017
Contact name Shamit Soneji
Organization name BMC
Street address Sölvegatan 19
City Lund
ZIP/Postal code 221 84
Country Sweden
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (16)
GSM1211399 Cont 0h rep1
GSM1211400 Cont 0h rep2
GSM1211401 Cont 0h rep3
This SubSeries is part of SuperSeries:
GSE49991 Dynamic analysis of gene expression and genome wide transcription factor binding during lineage-specification of multipotent progenitors
BioProject PRJNA215758

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49990_RAW.tar 35.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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