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Series GSE49987 Query DataSets for GSE49987
Status Public on Oct 17, 2013
Title Dynamic analysis of gene expression and genome wide transcription factor binding during lineage-specification of multipotent progenitors [TC]
Organism Mus musculus
Experiment type Expression profiling by array
Summary An expression time course experiment to investigate gene expression changes during differentiation of multipotential cells over two lineages using the model cell line FDCPmix differentiating towards erytroid and myeloid fates.
We used the paradigmatic 'GATA-PU.1 axis’ to explore, at systems-level, dynamic relationships between transcription factor (TF) binding and global gene expression programs as multipotent cells differentiate. We combined global ChIPSeq of GATA1, GATA2 and PU.1 with expression profiling during differentiation to erythroid and neutrophil lineages. Our analysis reveals (i) differential complexity of sequence motifs bound by GATA1, GATA2 and PU.1; (ii) the scope and interplay of the GATA1 and GATA2 programs within, and during transitions between, different cell compartments, and the extent of their hard-wiring by DNA motifs; (iii) the potential to predict gene expression trajectories based on global associations between TF-binding data and target gene expression and (iv) how dynamic modeling of DNA-binding and gene expression data can be used to infer regulatory logic of TF circuitry. This 'rubric' exemplifies the utility of this cross-platform resource for deconvoluting the complexity of transcriptional programs controlling stem/progenitor cell fate in hematopoiesis.
Overall design Multipotential FDCPmix cells were placed in two separate cytokine conditions and differentiated to erythroid and myeloid cells. Samples were taken at the time intervals indicated and hybridised to Agilent expression arrays. Each time point was done in triplicate.
Contributor(s) Enver T, Soneji S
Citation(s) 24120743
Submission date Aug 19, 2013
Last update date Jan 12, 2017
Contact name Shamit Soneji
Organization name BMC
Street address Sölvegatan 19
City Lund
ZIP/Postal code 221 84
Country Sweden
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (177)
GSM1211192 FDCP T0 rep1
GSM1211193 FDCP T0 rep2
GSM1211194 FDCP T0 rep3
This SubSeries is part of SuperSeries:
GSE49991 Dynamic analysis of gene expression and genome wide transcription factor binding during lineage-specification of multipotent progenitors
BioProject PRJNA215760

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49987_RAW.tar 1.4 Gb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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