|
Status |
Public on Dec 31, 2015 |
Title |
Transcriptome analysis of annotated coding genes and sORF under elevated CO2 |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
|
Summary |
There are seedling samples (high CO2 exposure of 0h, 2h, 6h, 12h, 1d, 3d, 7d, 14d) with duplicates in two chambers.
|
|
|
Overall design |
Arabidopsis WT (Col-0) seeds were plated on Murashige and Skoog plates and placed at 4°C in darkness for at least 2 d to synchronize germination. Plants were grown at 22C under long-day conditions (16-h light and 8-h dark) in two atmospheric CO2 environments: ambient (CO2: 390 μmol mol–1) or elevated (CO2: 780 μmol mol–1). Plants were grown in ambient atmospheric CO2 concentration and then exposed to elevated CO2 for 0 h, 2 h, 6 h, 12 h, 1 d, 3 d, 7 d and 14 d. 14-d-old seedlings were sampled at the same time. Treatments for elevated CO2 were carried out two times using two different chambers. Duplicate samples were collected from each chamber experiment. Totally, four biologically replicates were prepared in each condition.
|
|
|
Contributor(s) |
Higuchi-Takeuchi M, Shimizu M, Hanada K |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Aug 18, 2013 |
Last update date |
Jan 01, 2016 |
Contact name |
Kousuke Hanada |
E-mail(s) |
kohanada@bio.kyutech.ac.jp
|
Organization name |
Kyushu Institute Technology
|
Department |
Department of Bioscience and Bioinformatics
|
Street address |
680-4 Kawazu
|
City |
Iizuka |
State/province |
Fukuoka |
ZIP/Postal code |
820-8502 |
Country |
Japan |
|
|
Platforms (1) |
GPL14937 |
Agilent-022830 RIKEN_AT_sORF0902 |
|
Samples (32)
|
|
Relations |
BioProject |
PRJNA215689 |