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| Status |
Public on Aug 16, 2013 |
| Title |
Primary term human trophoblasts exposed to ionizing irradiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Primary term human trophoblasts were derived from placentas after a healthy pregnancy, and exposed to ionizing irradiation (vs sham) in vitro
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| Overall design |
Primary human trophoblasts were irradiated 24 h after initial plating, defined as time zero. Cells were irradiated at 10 Gy using a Clinac 600C (Varian Medical Systems, Palo Alto, CA) with a 6 MV photon beam and a dose rate of 250 cGy/min. The flasks containing the cells were placed on 1.5 cm of bolus (a tissue equivalent material) since the maximum irradiation depth was 1.5 cm, which corresponded to the plated cell layer. Cells were analyzed 4, 8, and 24 h after irradiation or sham.
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| Contributor(s) |
Sadovsky Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 15, 2013 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Yoel Sadovsky |
| E-mail(s) |
ysadovsky@mwri.magee.edu
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| Phone |
412-641-2675
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| Organization name |
MAGEE WOMENS RESEARCH INSTITUTE
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| Department |
Obstetrics and Gynecology
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| Lab |
Sadovsky
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| Street address |
204 Craft Ave
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| City |
PITTSBURGH |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (12)
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| GSM1209849 |
Trophoblasts, irradiated 8h placenta 1 |
| GSM1209851 |
Trophoblasts, control, 24h placenta 1 |
| GSM1209854 |
Trophoblasts, irradiated 24h placenta 1 |
| GSM1209855 |
Trophoblasts, control, 4h placenta 2 |
| GSM1209857 |
Trophoblasts, irradiated 4h placenta 2 |
| GSM1209859 |
Trophoblasts, control, 8h placenta 2 |
| GSM1209862 |
Trophoblasts, irradiated 8h placenta 2 |
| GSM1209864 |
Trophoblasts, control, 24h placenta 2 |
| GSM1209866 |
Trophoblasts, irradiated 24h placenta 2 |
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| This SubSeries is part of SuperSeries: |
| GSE49924 |
Irradiation of human and mouse placental cells |
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| Relations |
| BioProject |
PRJNA215352 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49922_RAW.tar |
42.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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