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| Status |
Public on Aug 15, 2013 |
| Title |
Genome-wide analysis of HL60 cells after 24h pulse of 10nM vincristine |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of HL60 response to low-dose vincristine at gene expression level. The hypothesis tested in the present study was that population heterogeneity has functional consequences in drug response. Since the presence of discrete efflux^High and efflux^Low subpopulations may reflect transitions between distinct stable cellular states (attractor states), we measured the transcriptomes when the cell population exhibited a stable bimodal distribution. We also measured the transcriptomes for 17 days after washing the drug (samples 5 - 9).
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| Overall design |
Total RNA obtained from HL60 cells untreated (sample 3), treated for 24h (sample 4) and sorted after 24h treatment for Calcein AM efflux low (sample 1) and high (sample 2). Sample 5 corresponds to total RNA of untreated cells, sample 6 to total RNA after 24h treatment with 10nM vincristine and samples 7 to 9 to total RNA after washing the drug.
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| Contributor(s) |
Pisco AO, Brock A, Zhou J, Moor A, Mojtahedi M, Jackson D, Huang S |
| Citation(s) |
24045430 |
| |
| Submission date |
Aug 14, 2013 |
| Last update date |
Oct 17, 2017 |
| Contact name |
Angela Oliveira Pisco |
| E-mail(s) |
angela.pisco@czbiohub.org
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| Organization name |
CZ Biohub
|
| Department |
Data Sciences
|
| Street address |
499 Illinois St
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94103 |
| Country |
USA |
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| Platforms (1) |
| GPL16807 |
Illumina HumanRef-8 WG-DASL v3.0 [gene-centered version] |
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| Samples (9)
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| GSM1208358 |
Mock sorted, treated |
| GSM1214248 |
Bulk population, day 0 (no drug) |
| GSM1214249 |
Bulk population, day 1 (24h 10nM vincristine) |
| GSM1214250 |
Bulk population, day 2 (drug washed at day 1) |
| GSM1214251 |
Bulk population, day 7 (drug washed at day 1) |
| GSM1214252 |
Bulk population, day 17 (drug washed at day 1) |
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| Relations |
| BioProject |
PRJNA215198 |
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