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Series GSE49826 Query DataSets for GSE49826
Status Public on Sep 17, 2013
Title Gene expression profiling on innervated and denervated muscles of Smad4 KO and control mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary To identify novel atrophy-related genes, which are controlled by BMP signaling, we performed gene expression profiling on innervated and 14 days denervated muscles of Smad4 knockout and control mice, focusing on genes that were differentially upregulated in denervated Smad4-/- muscles compared to controls. Among the different genes our attention was attracted by a gene that encodes for a novel f-box protein (Fbxo30) belonging to the SCF complex family of the ubiquitin ligases.
Cell size is determined by the balance between protein synthesis and degradation. This equilibrium is affected by hormones, nutrients, energy levels, mechanical stress and cytokines. Mutations that inactivate Myostatin lead to important muscle growth in animals and humans. However, the signals and pathways responsible for this hypertrophy remain largely unknown. Here we find that BMP signaling, acting through Smad1/5/8, is the fundamental hypertrophic signal. Inhibition of BMP signaling causes muscle atrophy, abolishes the hypertrophic phenotype of Myostatin knockout and strongly exacerbates the effects of denervation and fasting. BMP-Smad1/5/8 negatively regulates a novel gene (Fbxo30) that encodes an ubiquitin ligase, that is required for muscle loss. Collectively, these data identify a critical role for the BMP pathway in adult muscle maintenance, growth and atrophy.
Overall design Gene expression profiling on innervated and 14 days denervated muscles of Smad4 knockout and control mice. Three independent experiments were performed for each experimental condition using different animals for each experiment.
Contributor(s) Sandri M, Sartori R
Citation(s) 24076600
Submission date Aug 13, 2013
Last update date Jan 12, 2017
Contact name Marco Sandri
Organization name VIMM (Venetian Institute of Molecular Biology)
Street address via Orus,2
City Padua
ZIP/Postal code 35129
Country Italy
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM1207771 muscle WT 1 denervated 14 days
GSM1207772 muscle WT 1 control
GSM1207773 muscle WT 2 denervated 14 days
BioProject PRJNA215027

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49826_RAW.tar 95.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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