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Series GSE49820 Query DataSets for GSE49820
Status Public on Aug 14, 2013
Title Misregulation of the broad-range phospholipase C activity increases the susceptibility of Listeria monocytogenes to intracellular killing by neutrophils
Organism Mus musculus
Experiment type Expression profiling by array
Summary Listeria monocytogenes is a facultative intracellular bacterial pathogen that tightly regulates the activities of various virulence factors during infection. A mutant strain (the plcBΔpro mutant) that has lost the ability to control the activity of a phospholipase C (PC-PLC) is attenuated a hundred fold in mice. This attenuation is not due to a lack of bacterial fitness, but appears to result from a modified host response to infection. The transcriptomic pattern of immunerelated genes in infected macrophages indicated no differential response to wild-type L. monocytogenes vs the plcBΔpro mutant.
Overall design Cultures of bone marrow derived macrophages from BALB/c were infected with either wild type or mutant L. monocytogens for 3, 6, or 9 hrs. The macrophages were then collected and RNA isolated for microarray analysis of gene expression.
Contributor(s) Blank BS, AbiAbdallah DS, Park JJ, Nazarova EV, PavinskiBitar A, Maurer KJ, Marquis H
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Submission date Aug 13, 2013
Last update date Jan 12, 2017
Contact name Helene Marquis
Organization name Cornell University
Department Microbiology & Immunology
Street address Vet Medical Center, Room C5-169
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (24)
GSM1207687 BMM_wtLm_3hr_rep1
GSM1207688 BMM_wtLm_3hr_rep2
GSM1207689 BMM_wtLm_3hr_rep3
BioProject PRJNA215020

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Supplementary file Size Download File type/resource
GSE49820_RAW.tar 52.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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