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Series GSE49693 Query DataSets for GSE49693
Status Public on Jun 19, 2014
Title Replication timing of siRNA control and siRNA PolQRKO cells
Organism Homo sapiens
Experiment type Other
Summary The human A-family DNA polymerase θ (Pol q) is a large, multidomain enzyme whose physiological function is still unclear despite its in vitro translesion synthesis capacity in front of DNA damage and its involvement in some features of DNA repair after external stress. Here we present evidence that Pol q holds a novel role in the absence of external stress as a critical determinant of the replication timing program in human cells. Pol q binds to chromatin at early G1 and is required for proper formation of pre-replicative complexe and replication origin activation. Pol q-depleted cells show modified spatial organization of chromatin-loop structures at replication factories. Genome-wide analysis of replication timing shows delayed replication of a part of early replicating domains and advanced replication of a part of late replicating domains following Pol q depletion. Our results identify Pol q as one of the first critical human factors discovered in the replication timing programme.
Overall design Two-condition experiment, siRNA control vs. siRNA polQ cells. Biological replicates: 2 control replicates, 2 transfected replicates.
Contributor(s) Fernandez-Vidal A, Guitton-Sert L, Cadoret J, Baldacci G, Cazaux C, Hoffmann J
Citation(s) 24989122
Submission date Aug 09, 2013
Last update date Sep 19, 2014
Contact name Jean-Charles Cadoret
Organization name CNRS/ Université de Paris
Street address 15 rue hélène Brion
City Paris
ZIP/Postal code 75013
Country France
Platforms (1)
GPL10123 Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version)
Samples (4)
GSM1204982 siRNA control replication timing 1
GSM1204983 siRNA control replication timing 2
GSM1204984 siRNA polQ replication timing 1
BioProject PRJNA214750

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Supplementary file Size Download File type/resource
GSE49693_RAW.tar 74.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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