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Series GSE49380 Query DataSets for GSE49380
Status Public on Aug 31, 2013
Title Whole genome expression analysis of tumor regulatory T cells expressing interleukin-10
Organism Mus musculus
Experiment type Expression profiling by array
Summary The tumor microenvironment contains high frequencies of inflammatory regulatory T (Treg) cells. These Treg exhibit superior regulatory function compared with those from other environments such as the spleen, partially due to expression of anti-inflammatory interleukin-10. In order to gain insight into the origins and functional roles of different Treg subsets, we used whole genome microarray analysis to characterize tumor IL-10+ and tumor IL-10- Treg subsets obtained from VERT-X reporter mice bearing transplantable tumors, along with total tumor Treg and spleen Treg from FoxP3-EGFP reporter mice. Few genes were found to differ between IL-10+ and IL-10- tumor Treg subsets (29 upregulated, 88 downregulated), suggesting a common origin of each Treg subset. The specific gene expression profile of IL-10+ tumor Treg was associated with the tumor microenvironment and absent from spleen Treg, suggesting it to be driven by components of the inflammatory tumor microenvironment. The IL-10+ tumor Treg gene expression profile displayed upregulation of genes associated with a higher activation state and greater effector function.
Overall design Pooled MC38 tumor tissue from VERT-X or FoxP3-EGFP reporter mice were used to obtain IL10+ tumor Treg (VERT-X), IL10- tumor Treg (VERT-X) and total tumor Treg (FoxP3-EGFP). Spleens from tumor-free FoxP3-EGFP mice were used for spleen Treg. Three experiments for each population gave a total 12 RNA samples.
Contributor(s) Stewart CA, Trinchieri G
Citation(s) 24216477
Submission date Jul 30, 2013
Last update date Jan 12, 2017
Contact name Charles Andrew Stewart
Organization name National Cancer Institute
Department Center For Cancer Research
Lab Cancer and Inflammation Program
Street address 1050 Boyles Street
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM1198579 MC38TumorTreg_VERTX_IL10neg_rep1
GSM1198580 MC38TumorTreg_VERTX_IL10pos_rep1
GSM1198581 MC38TumorTreg_VERTX_IL10neg_rep2
BioProject PRJNA215307

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Supplementary file Size Download File type/resource
GSE49380_RAW.tar 96.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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