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| Status |
Public on Apr 04, 2016 |
| Title |
Gene expression in haematopoietic stem and progenitor cells from Gata1-EGFP reporter mouse bone marrow |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Haematopoietic stem cells can differentiate into all blood cell types. In this process, cells become progressively restricted to a single cell type. The order in which differentiating cells loose lineage potential, and the prospective isolation of cells with a defined potential remains a long-standing question.
We performed gene expression analysis of haematopoietic cells from Gata1-EGFP reporter mice, leading to a model for hematopoiesis where the initial lineage decision consists of a separation of erythroid/megakaryocyte/mast cell/eosinophil potential from lymphopoietic/monocyte/neutrophil potential
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| Overall design |
RNA was isolated from HSC EGFP-, HSC EGFP+, LMPP, CLP, preGM EGFP-, preGM EGFP+, GMP EGFP-, GMP EGFP+ and preMegE cells with the QIAGEN RNeasy Micro Kit.
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| Contributor(s) |
Drissen R, Buza-Vidas N, Mead A, Mancini E, Lutteropp M, Grover A, Jacobsen SW, Nerlov C |
| Citation(s) |
27043410 |
| |
| Submission date |
Jul 26, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Claus Nerlov |
| E-mail(s) |
claus.nerlov@imm.ox.ac.uk
|
| Organization name |
Weatheral Institute of Molecular Medicine
|
| Department |
MRC Molecular Haematology Unit
|
| Lab |
Nerlov Laboratory
|
| Street address |
John Radcliffe Hospital, University of Oxford
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA213377 |
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