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Series GSE49137 Query DataSets for GSE49137
Status Public on May 16, 2014
Title INO80 complex in the core regulatory network governing ESC self-renewal [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Embryonic stem cell (ESC) self-renewal and pluripotency is controlled by the coordinated action of transcription factors and chromatin regulators. Compared to the pluripotency transcription factors, the function of the chromatin regulators, especially the ATP-dependent chromatin remodelers, remains poorly understood in ESCs. Here, we show that INO80, a SWI/SNF family chromatin remodeling complex, is essential for ESC self-renewal, pluripotency, somatic cell reprogramming, and embryonic development. Ino80, the ATPase of the complex, forms an auto-regulatory loop with the ESC master transcription factors Oct4, Nanog, and Sox2. More importantly, it co-occupies the enhancer regions of most key pluripotency genes with the master transcription factors, and positively regulates their expression by maintaining an open chromatin structure. Our data suggests that INO80 is an integral component of the pluripotency transcription network, and plays a critical role in both the maintenance and establishment of pluripotency
Overall design Identification of Ino80 localization in mouse embryonic stem cells
Contributor(s) Wang L, Hu G, Du Y
Citation(s) 24792115
Submission date Jul 23, 2013
Last update date May 15, 2019
Contact name Li Wang
Organization name NIH
Street address 111 T.W. Alexander Dr
City Durham
ZIP/Postal code 27709
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (12)
GSM1194194 J1_Ino80
GSM1194195 J1_Ino80_input
GSM1234308 J1_NT_input
This SubSeries is part of SuperSeries:
GSE49252 INO80 complex in the core regulatory network governing ESC self-renewal
BioProject PRJNA213131
SRA SRP028171

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49137_J1_Ino80_CalledPeaks.bed.gz 122.2 Kb (ftp)(http) BED
GSE49137_RAW.tar 1.2 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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