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Series GSE49064 Query DataSets for GSE49064
Status Public on May 09, 2014
Title Aging-induced differential methylation in human PBMCs occurs with but often without change in expression of the associated gene (DNA Methylation)
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary Aging is a progressive process that results in the accumulation of intra- and extracellular alterations that in turn contribute to a reduction in health. Age-related changes in DNA methylation have been reported before and may be responsible for aging-induced changes in gene expression, although a causal relationship has yet to be shown. Using genome-wide assays, we analyzed age-induced changes in DNA methylation and their effect on gene expression with and without transient induction with the synthetic transcription modulating agent WY14,643. To demonstrate feasibility of the approach, we isolated peripheral blood mononucleated cells (PBMCs) from five young and five old healthy male volunteers and cultured them with or without WY14,643. Infinium 450K BeadChip and Affymetrix Human Gene 1.1 ST expression array analysis revealed significant differential methylation of at least 5 % (ΔYO > 5 %) at 10,625 CpG sites between young and old subjects, but only a subset of the associated genes were also differentially expressed. Age-related differential methylation of previously reported epigenetic biomarkers of aging including ELOVL2, FHL2, PENK, and KLF14 was confirmed in our study, but these genes did not display an age-related change in gene expression in PBMCs. Bioinformatic analysis revealed that differentially methylated genes that lack an age-related expression change predominantly represent genes involved in carcinogenesis and developmental processes, and expression of most of these genes were silenced in PBMCs. No changes in DNA methylation were found in genes displaying transiently induced changes in gene expression. In conclusion, aging-induced differential methylation often targets developmental genes and occurs mostly without change in gene expression.
Overall design Peripheral blood mononuclear cells were isolated from 10 volunteers (5 young 5 old), treated with the synthetic PPARalpha agonists WY14,643 or control for 13 hrs, and subjected to genome-wide DNA methylation and gene expression analysis. This entry contains the DNA methylation data.
Contributor(s) Steegenga WT, Morris T, Boekschoten MV, Hooiveld GJ, Müller M
Citation(s) 24789080
Submission date Jul 19, 2013
Last update date Mar 22, 2019
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (20)
GSM1193238 PBMC_Ctrl_subj1
GSM1193239 PBMC_Ctrl_subj2
GSM1193240 PBMC_Ctrl_subj3
This SubSeries is part of SuperSeries:
GSE49065 Aging-induced differential methylation in human PBMCs occurs with but often without change in expression of the associated gene
BioProject PRJNA215867

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49064_RAW.tar 183.1 Mb (http)(custom) TAR
GSE49064_group_assignment_methylation_data.txt.gz 364 b (ftp)(http) TXT
GSE49064_unmethylated_methylated_raw_data.txt.gz 86.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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