GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE48966 Query DataSets for GSE48966
Status Public on Mar 16, 2015
Title Liver Med23 ablation improves glucose and lipid metabolism and prevent diet-induced obesity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mediator complex function as an integrative hub for transcriptional regulation. Here we show that Mediator subunit MED23 regulate glucose and lipid metabolism via FOXO1 in liver. Here, we have generated a liver-specific Med23-knockout (LMKO) mouse and found that Med23-deletion in liver improved glucose and lipid metabolism, as well as insulin responsiveness, and prevented diet-induced obesity. Mechanistically, MED23 participated in gluconeogenesis and cholesterol synthesis by interacting with FOXO1. Disruption of this interaction by hepatic Med23-deletion impaired the Mediator and RNAP II recruitment and partially reduced the expression of the FOXO1 target genes. Remarkably, acute hepatic Med23 knockdown in db/db mice significantly improved insulin sensitivity. Overall, our data revealed Mediator MED23 as a critical regulator of glucose and lipid metabolism, suggesting novel therapeutic strategies against metabolic diseases.
Overall design Comparison of mRNA expression profile of control and LMKO mice fasted 24 h (fasted) or fasted 24 h then re-fed 24 h (re-fed) (n = 2 per group)
Contributor(s) Chu Y, Xu Y, Yao X, Wang G
Citation(s) 25223702
Submission date Jul 17, 2013
Last update date May 15, 2019
Contact name Yajing Chu
Phone 86-22-23909396
Organization name Institute of Hematology, Chinese Academy of Medical Sciences
Department State Key Laboratory of Experimental Hematology
Lab Weiping Yuan Lab
Street address Nanjing Road 288
City Tianjin
ZIP/Postal code 300020
Country China
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM1188117 Wang_ctrl1_fasted_RNASeq
GSM1188120 Wang_ctrl2_fasted_RNASeq
GSM1188121 Wang_LMKO1_fasted_RNASeq
BioProject PRJNA212436
SRA SRP027536

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48966_RAW.tar 3.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap