|
| Status |
Public on Mar 16, 2015 |
| Title |
Liver Med23 ablation improves glucose and lipid metabolism and prevent diet-induced obesity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mediator complex function as an integrative hub for transcriptional regulation. Here we show that Mediator subunit MED23 regulate glucose and lipid metabolism via FOXO1 in liver. Here, we have generated a liver-specific Med23-knockout (LMKO) mouse and found that Med23-deletion in liver improved glucose and lipid metabolism, as well as insulin responsiveness, and prevented diet-induced obesity. Mechanistically, MED23 participated in gluconeogenesis and cholesterol synthesis by interacting with FOXO1. Disruption of this interaction by hepatic Med23-deletion impaired the Mediator and RNAP II recruitment and partially reduced the expression of the FOXO1 target genes. Remarkably, acute hepatic Med23 knockdown in db/db mice significantly improved insulin sensitivity. Overall, our data revealed Mediator MED23 as a critical regulator of glucose and lipid metabolism, suggesting novel therapeutic strategies against metabolic diseases.
|
| |
|
| Overall design |
Comparison of mRNA expression profile of control and LMKO mice fasted 24 h (fasted) or fasted 24 h then re-fed 24 h (re-fed) (n = 2 per group)
|
| |
|
| Contributor(s) |
Chu Y, Xu Y, Yao X, Wang G |
| Citation(s) |
25223702 |
| Submission date |
Jul 17, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Yajing Chu |
| E-mail(s) |
chuyajing@ihcams.ac.cn
|
| Phone |
86-22-23909396
|
| Organization name |
Institute of Hematology, Chinese Academy of Medical Sciences
|
| Department |
State Key Laboratory of Experimental Hematology
|
| Lab |
Weiping Yuan Lab
|
| Street address |
Nanjing Road 288
|
| City |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA212436 |
| SRA |
SRP027536 |
Less...
More...