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Series GSE48486 Query DataSets for GSE48486
Status Public on Jul 19, 2013
Title Reprogramming fibroblasts into bi-potential hepatic stem cells by defined factors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Recent studies have demonstrated direct reprogramming of fibroblasts into a range of somatic cell types, but to date stem/progenitor cells have only been reprogrammed for the blood and neuronal lineages. We previously reported generation of induced hepatocyte-like (iHep) cells by transduction of Gata4, Hnf1α, and Foxa3 in p19 Arf null mouse embryonic fibroblasts (MEFs). Here, we show that Hnf1β and Foxa3, liver organogenesis transcription factors, are sufficient to reprogram MEFs into induced hepatic stem cells (iHepSCs). iHepSCs can be stably expanded in vitro and possess the potential of bi-directional differentiation into both hepatocytic and cholangiocytic lineages. In the injured liver of fumarylacetoacetate hydrolase (Fah)-deficient mice, repopulating iHepSCs become hepatocyte-like cells. They also engraft as cholangiocytes into bile ducts of mice with DDC-induced bile ductular injury. Lineage-conversion into bi-potential expandable iHepSCs provides a strategy to enable efficient derivation of both hepatocytes and cholangiocytes for use in disease modeling and tissue engineering.
Overall design iHepSCs were converted form fibroblasts by transduction of Hnf1β and Foxa3. iHepSCs were induced to differentiate into hepatocyte-like cells and cholangiocytes in vitro. Totally, 9 samples including four clones of iHepSCS, one clone of LEPCs, two samples of MEFs and two samples of iHepSCs-derived cholangocytes were analyzed.
Contributor(s) Yu B, He Z, You P, Han Q, Xiang D, Chen F, Wang M, Liu C, Lin X, Borjigin U, Zi X, Li J, Zhu H, Li W, Han C, Wangensteen KJ, Shi Y, Hui L, Wang X, Hu Y
Citation(s) 23871605
Submission date Jul 02, 2013
Last update date Jan 12, 2017
Contact name Pu You
Organization name Second Military Medical University
Street address 800 Xiangyin Road
City Shanghai
ZIP/Postal code 200433
Country China
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (9)
GSM1179795 inducedHepSC_rep1
GSM1179796 inducedHepSC_rep2
GSM1179797 inducedHepSC_rep3
BioProject PRJNA210316

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48486_RAW.tar 19.7 Mb (http)(custom) TAR (of TXT)
GSE48486_normalized_data.txt.gz 1.8 Mb (ftp)(http) TXT
Processed data are available on Series record

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