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| Status |
Public on Dec 24, 2013 |
| Title |
Expression data from ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
H2A.B is a unique histone H2A variant that shares only 40 ~ 50 % sequence identity with canonical H2A. It has only been identified in mammals and has quickly evolved with remarkable sequence diversity among different species. H2A.B is ubiquitously expressed in most cells and tissues. It is mainly deposited in gene body region.The localization of H2A.B is associated with methylated CpG islands in mouse ES cells.H2A.B facilitates transcription elongation to go through methylated CpG islands in the gene bodies. One typical example is that H2A.B regulates transcription elongation at imprinted loci.
We used microarray to test the function of H2A.B on gene expression.
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| Overall design |
Mouse ES cells infected with control knockdown(KD) or H2A.B KD virus were treated with G418. ES cells were extracted for RNA and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Chen Y, Chen Q, Yu X |
| Citation missing |
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| Submission date |
Jul 01, 2013 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Yibin Chen |
| E-mail(s) |
yibin@umich.edu
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| Organization name |
university of Michigan
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| Lab |
Xiaochun Yu
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| Street address |
1115 w medical drive
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48104 |
| Country |
USA |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE49294 |
H2A.B facilitates transcription at methylated CpG Loci |
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| Relations |
| BioProject |
PRJNA210153 |
