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Series GSE48364 Query DataSets for GSE48364
Status Public on Sep 12, 2013
Title Transcriptional profiles by deep sequencing (RNA-seq) of in vivo-generated mouse iPSCs, in vitro-generated mouse iPSCs, and mouse ESCs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have generated “reprogrammable” transgenic mice that ubiquitously express the four Yamanaka factors in an inducible manner. Transitory induction of the transgene results in multiple teratomas emerging from a variety of organs, thus indicating that full reprogramming into iPSCs can occur in vivo. By performing bone marrow transplant experiments, we demonstrate that both hematopoietic cells, as well as non-hematopoietic cells can be reprogrammed in vivo. Remarkably, reprogrammable mice also present circulating iPSCs in the bloodstream (in vivo-iPSCs) with all the expected properties of bona fide iPSCs. Moreover, in contrast to in vitro-iPSCs or embryonic stem cells (ESCs), in vivo-iPSCs have an increased capacity to undergo trophectoderm lineage differentiation, which suggests that in vivo-iPSCs are more plastic or primitive than in vitro-generated iPSCs or ESCs.
Overall design 6 clones of in vivo-generated iPSCs, 5 indendent clones of in vitro-generated iPSCs, and 3 clones of established ESCs
Contributor(s) Abad M, Graña O, Serrano M
Citation(s) 24025773
Submission date Jun 27, 2013
Last update date May 15, 2019
Contact name Manuel Serrano
Organization name IRB
Department Molecular Medicine
Lab Cellular Plasticity and Disease
Street address Parc Científic de Barcelona, C/ Baldiri Reixac 10
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (14)
GSM1176461 in vivo iPSC clone 12708-1
GSM1176462 in vivo iPSC clone 12708-6
GSM1176463 in vivo iPSC clone 12708-7
BioProject PRJNA209846
SRA SRP026364

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48364_RAW.tar 10.8 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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