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Series GSE48206 Query DataSets for GSE48206
Status Public on Oct 27, 2013
Title Gene Expression Profile for knocking down Tet1 in pre-iPSCs culturing in Vc medium.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation. As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones, it remains unclear the role of Tet1 in the reprogramming process. Here, we performed RNAseq for WT and knocking down Tet1 in two pre-iPSCs cell lines, pre2-2 and pre3.
Overall design Using two pre-iPSCs cell lines: pre2-2 and pre3, cultured by mES medium(+lif), transfected siCtrl/siTet1 siRNA or non-treatment, then, after 16h, the medium were replaced by mES(+lif) medium plus Vc(50ug/ml), total RNA was extracted after another 32h later for RNAseq.
Contributor(s) Pei D, Xu G
Citation(s) 24162740
Submission date Jun 21, 2013
Last update date May 15, 2019
Contact name Lihui Lin
Phone 020-32015291
Organization name Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
Department Stem cell
Lab Pei Duanqing
Street address No.190, Kaiyuan Street
City Guangzhou
State/province Guangdong
ZIP/Postal code GD20
Country China
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (6)
GSM1172734 pre2-2_NT
GSM1172735 pre2-2_NC
GSM1172736 pre2-2_siTet1
BioProject PRJNA209199
SRA SRP026238

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48206_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
GSE48206_all_samples.tpm.txt.gz 225.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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