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Series GSE48042 Query DataSets for GSE48042
Status Public on Jun 18, 2013
Title Transcriptome analysis of the early response to nickel (Ni) in Acidiphilium sp. PM
Organism Acidiphilium sp. PM
Experiment type Expression profiling by array
Summary To study the mechanisms of Ni resistance in the metal resistant Acidiphilium sp. PM, the transcriptome of Acidiphilium sp. PM was studied 5min and 30 min after the addition of 10mM Ni and compared to the transcriptome in untreated cells.
Overall design Two-condition hybridization experiments: untreated cells vs cells treated with 10mM Ni (for either 5 or 30 minutes). Eighteen 100ml-cultures: 6 untreated control cultures (not exposed to Ni), 6 cultures exposed to 10mM Ni for 5min, and 6 cultures exposed to 10mM Ni for 30 min. Cultures were independently grown and harvested. Gene expression levels of one untreated replicate were compared separately with one 5min-exposed replicate and with one 30min-exposed replicate (yielding two microarray data sets. One for each hybridization).
Contributor(s) San Martin-Uriz P, Alcolea PJ, Moreno-Paz M, Gomez MJ, Amils R
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Submission date Jun 17, 2013
Last update date Jun 20, 2013
Contact name Patxi San Martin-Uriz
Organization name Instituto Nacional de Tecnica Aeroespacial
Department Centro de Astrobiologia
Street address Ctra. de Ajalvir, km. 4
City Torrejon de Ardoz
State/province Madrid
ZIP/Postal code 28850
Country Spain
Platforms (1)
GPL17306 Centro de Astrobiologia_Acidiphilium sp. PM shotgun genomic DNA microarray_17184 spots_v1.0
Samples (12)
GSM1166219 Apm_5minNi_rep1
GSM1166220 Apm_5minNi_rep2
GSM1166221 Apm_5minNi_rep3
BioProject PRJNA208748

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48042_RAW.tar 28.8 Mb (http)(custom) TAR (of GPR, TXT)
Processed data included within Sample table

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