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| Status |
Public on Dec 25, 2013 |
| Title |
An epigenetic profile of early T-cell development from multipotent progenitors to committed T-cell descendants. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Using a stromal cell free system, we described the gene expression and two genome wide epigenetic profiles of a unique population of undifferentiated bone marrow cells selectively driven towards the T cell differentiation pathway
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| Overall design |
Undifferentiated bone marrow cells, transduced with NUP98-HOXB4 fusion protein, in vitro expanded with IL6 and SCF represents the hematopoietic stem cells (HSC); when grown in the presence of DLL4 (Notch1 ligand) and IL7, they are directed towards the T cell lineage (ProT); these in vitro generated ProT cells injected in a Rag2-/- mouse give rise to mature double positive T cells (DP). 2 replica for each population
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| Contributor(s) |
Vigano MA, Ivanek R |
| Citation(s) |
24374622 |
| Submission date |
Jun 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
maria alessandra vigano |
| E-mail(s) |
alessandra.vigano@unibas.ch
|
| Organization name |
university of basel
|
| Department |
department of biomedicine
|
| Street address |
mattenstrasse,28
|
| City |
basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA208606 |
| SRA |
SRP026079 |
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