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Series GSE47995 Query DataSets for GSE47995
Status Public on Dec 25, 2013
Title An epigenetic profile of early T-cell development from multipotent progenitors to committed T-cell descendants.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Using a stromal cell free system, we described the gene expression and two genome wide epigenetic profiles of a unique population of undifferentiated bone marrow cells selectively driven towards the T cell differentiation pathway
Overall design Undifferentiated bone marrow cells, transduced with NUP98-HOXB4 fusion protein, in vitro expanded with IL6 and SCF represents the hematopoietic stem cells (HSC); when grown in the presence of DLL4 (Notch1 ligand) and IL7, they are directed towards the T cell lineage (ProT); these in vitro generated ProT cells injected in a Rag2-/- mouse give rise to mature double positive T cells (DP). 2 replica for each population
Contributor(s) Vigano MA, Ivanek R
Citation(s) 24374622
Submission date Jun 16, 2013
Last update date May 15, 2019
Contact name maria alessandra vigano
Organization name university of basel
Department department of biomedicine
Street address mattenstrasse,28
City basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (15)
GSM1164633 HSC_H3K4me3_rep1
GSM1164634 HSC_H3K4me3_rep2
GSM1164635 HSC_H3K27me3_rep1
BioProject PRJNA208606
SRA SRP026079

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47995_RAW.tar 587.0 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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