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| Status |
Public on Jun 15, 2013 |
| Title |
RNA-seq analysis of global RNA levels at 4 stages of directed cardiac differentiation of mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We interrogated the transcriptome using RNA-seq at several stages of an mouse embryonic stem cell to cardiomyocyte directed differentiation protocol. These four stages represent timepoints when differentiating cultures are enriched for embryonic stem cells (ESC), mesodermal cells (MES), cardiac precursors (CP), or cardiomyocytes (CM) respectively. This study revealed many dynamic patterns of mRNAs and long non-coding RNAs (lncRNAs) and identified groups of genes with similar expression patterns during differentiation.
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| Overall design |
RNA-seq analysis of global RNA levels at 4 stages of directed cardiac differentiation of mouse embryonic stem cells. Each stage in biological duplicates
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| Contributor(s) |
Alexander J, Wamstad J |
| Citation(s) |
22981692 |
| Submission date |
Jun 14, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Laurie A Boyer |
| E-mail(s) |
lboyer@mit.edu
|
| Phone |
617 324-3335
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Boyer
|
| Street address |
77 Massachusetts Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE47950 |
Dynamic and Coordinated Epigenetic Regulation of Developmental Transitions in the Cardiac Lineage |
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| Relations |
| BioProject |
PRJNA208437 |
| SRA |
SRP026035 |
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