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Series GSE47877 Query DataSets for GSE47877
Status Public on Jul 15, 2014
Title Transcriptome analysis of Germinal Center and naïve B cells from miR-217TG and control mice by RNAseq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary microRNAs (miRNAs) regulate virtually all biological processes, but little is known of their role in germinal center (GC) B cells. While the GC reaction is crucial to ensure a competent immune response, GC B cells are also the origin of most human lymphomas. Here we report that miR-217 is specifically upregulated in GC B cells. Gain- and loss-of-function mouse models reveal that miR-217 functions as a positive modulator of the GC response through the regulation of a DNA repair gene network. Moreover, we show that miR-217 overexpression promotes mature B cell lymphomagenesis. Therefore miR-217 provides a novel molecular link between the normal GC response and B cell transformation
Overall design 4 samples were analyzed by RNAseq: 1) naïve (CD19+Fas-GL7-) B cells from miR-217TG, 2) GC (CD19+Fas+GL7+) B cells from miR-217TG, 3) naïve (CD19+Fas-GL7-) B cells from littermate controls and 4) GC (CD19+Fas+GL7+) B cells from littermate controls. Samples were isolated by cell sorting from pooled Peyer’s patches (4-6 animals per genotype). Two independent experiments were performed.
Contributor(s) de Yébenes VG, Bartolomé-Izquierdo N, Nogales-Cadenas R, Pérez-Durán P, Martínez N, Robbiani D, Cañamero M, Piris MA, Pascual-Montano A, Ramiro AR
Citation(s) 24850757
Submission date Jun 12, 2013
Last update date May 15, 2019
Contact name Almudena Ramiro
Organization name Centro Nacional de Investigaciones Cardiovasculares
Department Vascular Biology and Inflammation
Lab B Cell Biology Lab
Street address Melchor Fernandez Almagro 3
City Madrid
ZIP/Postal code 28029
Country Spain
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM1161545 control_CD19_DN_R1
GSM1161546 control_CD19_DN_R2
GSM1161547 control_CD19_FasGl7_R1
BioProject PRJNA208231
SRA SRP025986

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Supplementary file Size Download File type/resource
GSE47877_RAW.tar 870.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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