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Series GSE47605 Query DataSets for GSE47605
Status Public on Mar 02, 2017
Title Differentiation of human and murine induced pluripotent stem cells to microglia-like cells [mouse]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Microglia are the resident inflammatory cells of the central nervous system (CNS) and have important roles in development, homeostasis and a variety of neurologic and psychiatric diseases. Difficulties in procuring human microglia have limited their study and hampered the clinical translation of microglia-based treatments shown to be effective in animal disease models. Here, we report the differentiation of human induced pluripotent stem cells (iPSC) into microglia-like cells by exposure to defined factors and co-culture with astrocytes. These iPSC-derived microglia (iPS-MG) have the phenotype, gene expression profile and functional properties of brain-isolated microglia. Murine iPS-MG generated using a similar protocol have equivalent efficacy to primary brain-isolated microglia in the treatment of murine syngeneic intracranial malignant gliomas. The ability to generate human microglia facilitates the further study of this important CNS cell type and raises the possibility of their use in personalized medicine applications.
Overall design 24 Mouse samples: 3 iPSC (induced pluripotent stem cell), 3 iPS-MG (GFP+ iPSC-derived microglia), 3 neo-MG (neonatal microglia), 3 ad-MG (adult microglia), 3 DC (dendritic cell), 3 NSC (neural stem cell), 3 bm-Mac (bone marrow macrophage), 3 p-Mac (peritoneal macrophage).
Contributor(s) Pandya H, Shen MJ, Ichikawa DM, Sedlock AB, Choi Y, Johnson KR, Kim G, Brown M, Elkhaloun A, Maric D, McGavern DB, Malech HL, Park JK
Citation(s) 28253233
Submission date Jun 03, 2013
Last update date Apr 18, 2017
Contact name Kory R Johnson
Phone 301-402-1956
Organization name NINDS/NIH
Department DIR IT & Bioinformatics
Lab Bioinformatics Section
Street address 10/3B01, 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (24)
GSM1153099 iPSC Replicate 1
GSM1153100 iPSC Replicate 2
GSM1153101 iPSC Replicate 3
This SubSeries is part of SuperSeries:
GSE78116 Differentiation of human and murine induced pluripotent stem cells to microglia-like cells
BioProject PRJNA206513

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47605_RAW.tar 109.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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