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Series GSE47555 Query DataSets for GSE47555
Status Public on Jun 01, 2013
Title Microarray approach to investigate gene expression in human ovarian tissue after xenografting
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cryobanking and transplantation of ovarian tissue is a promising approach to restore fertility in cancer patients. However, ischemic stress following avascular ovarian cortex grafting is known to induce stromal tissue fibrosis and alteration in follicular development.
The aim of the study is to analyse the impact of freezing-thawing and grafting procedures on gene expression in human ovarian tissue. Frozen-thawed ovarian tissue from 4 patients was xenografted for 7 days in nude mice and one non-grafted fragment was used as control.
Immediately after recovery, grafts were processed for RNA extraction and histological analysis. Their expression profile was screened by whole-genome oligonucleotide array (n=4) and validated by reverse-transcriptase polymerase chain analysis (n=10).
84 of the transcripts were significantly altered after 7 days of grafting including matrix metalloproteinase-9 and -14 and angiogenic factors such as Placental growth factor and C-X-C chemokine receptor type 4 (CXCR4). Major biological processes were related to tissue remodelling including secretory processes, cellular adhesion and response to chemical and hormone stimuli.
Angiopoietin signaling, interleukin-8 pathway and peroxisome proliferator-activated receptors activation were shown to be differentially regulated. On day 7, overexpression was confirmed by PCR for interleukin-8, transforming growth factor-beta 1, matrix metalloproteinase-14 and CXCR4 compared to the nongrafted control.
In conclusion, new as well as known genes involved in tissue restructuration and angiogenesis were identified after human ovarian tissue transplantation. This will facilitate the development of strategies to optimize grafting techniques.
 
Overall design In this study, ovarian tissue was obtained from 4 patients. Each biopsy was divided into 2 fragments. One fragment was frozen/thawed and placed in TRIzol reagent (Invitrogen) for RNA extraction. The other fragment was frozen-thawed and grafted in the intraperitoneal cavity of a nude mice. After 7 days graft were recovered and total RNA was extracted.
 
Contributor(s) Ambroise J, Bearzatto B, Van Langendonckt A, Romeu L, Amorim C, Gala JL, Donnez J, Dolmans MM
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Submission date May 31, 2013
Last update date Dec 06, 2018
Contact name Jérôme Ambroise
E-mail(s) jerome.ambroise@uclouvain.be
Organization name Université catholique de Louvain
Department Institut de Recherche Expérimentale et Clinique (IREC)
Lab Centre des Technologies Moléculaires Appliquées (CTMA)
Street address 30, Chapelle-aux-Champs (box B1.30.24)
City Woluwé-St-Lambert
ZIP/Postal code 1200
Country Belgium
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM1152400 Ovariant tissue harvest at Day 0, Patient 1
GSM1152401 Ovariant tissue harvest at Day 7, Patient 1
GSM1152402 Ovariant tissue harvest at Day 0, Patient 2
Relations
BioProject PRJNA206073

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47555_RAW.tar 16.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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