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Series GSE47528 Query DataSets for GSE47528
Status Public on Jun 01, 2014
Title Jarid2, a Polycomb member and target of miR-155, regulates the fates of Th17 and Treg cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Genome-wide analysis of Jarid2, Suz12, and c-Maf binding and H3K27me3 profiling in miR-155 KO and WT Th17 performed by ChIP-seq. We found that Jarid2 and c-Maf is differentially expressed in absence of miR-155 and they compete for binding to the Il22 promoter. We highlight targets of Jarid2 and Suz12 in miR-155 KO Th17 cells that are epigenetically silenced by increased H3K27me3 status. Furthermore, genome-wide analysis through Suz12 ChIP-exo in WT and Jarid2fl/fl;CD4cre Th17 reveals defects in PRC2 recruitment in abscence of Jarid2 that results in derepression of genes in Th17 cells. Thus, one main function of miR-155 is to curb epigenetic silencing by targeting Jarid2.
Overall design Examination of Jarid2, Suz12, c-Maf binding and H3K27me3 changes in miR-155 KO and WT Th17.
Contributor(s) Kilaru GK, Escobar TM, Muljo SA
Citation(s) 24856900
Submission date May 30, 2013
Last update date May 15, 2019
Contact name Gokhul Krishna Kilaru
Phone 214-648-4158
Organization name University of Texas Southwestern Medical Center
Department Department of Neuroscience
Lab Joseph S. Takahashi
Street address 6000 Harry Hines Boulevard
City Dallas
State/province TEXAS
ZIP/Postal code 75390
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (12)
GSM1151615 Input_DNA-miR155-WT
GSM1151616 Input_DNA-miR155-KO
GSM1151617 Jarid2_ChIPSeq-miR155-WT
BioProject PRJNA205981
SRA SRP023466

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Supplementary file Size Download File type/resource
GSE47528_RAW.tar 543.2 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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