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Series GSE47512 Query DataSets for GSE47512
Status Public on Nov 15, 2013
Title DNA methylation analysis of subcutaneous adipose tissue abdominal versus subcutenaous adipose tissue gluteal
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary In humans, adipose tissue is distributed in subcutaneous abdominal and subcutaneous gluteal depots that comprise a variety of functional differences. Whereas energy storage in gluteal adipose tissue has been shown to mediate a protective effect, an increase of abdominal adipose tissue is associated with metabolic disorders. However, the molecular basis of depot-specific characteristics is not completely understood yet. Using array-based analyses of transcription profiles, we identified a specific set of genes that was differentially expressed between subcutaneous abdominal and gluteal adipose tissue. To investigate the role of epigenetic regulation in depot-specific gene expression, we additionally analyzed genome-wide DNA methylation patterns in abdominal and gluteal depots. By combining both data sets, we identified a highly significant set of depot-specifically expressed genes that appear to be epigenetically regulated. Interestingly, the majority of these genes form part of the homeobox gene family. Moreover, genes involved in fatty acid metabolism were also differentially expressed. Therefore we suppose that changes in gene expression profiles might account for depot-specific differences in lipid composition. Indeed, triglycerides and fatty acids of abdominal adipose tissue were more saturated compared to triglycerides and fatty acids in gluteal adipose tissue. Taken together, our results uncover clear differences between abdominal and gluteal adipose tissue on the gene expression and DNA methylation level as well as in fatty acid composition. Therefore, a detailed molecular characterization of adipose tissue depots will be essential to develop new treatment strategies for metabolic syndrome associated complications.
 
Overall design DNA methylation profiles of abdominal adipose tissue (6 samples) and gluteal adipose tissue (6 samples) were generated using Infinium methylation 450K BeadChips from Illumina (Illumina, San Diego, USA).
 
Contributor(s) Gehrke S, Winnefeld M, Hagemann S
Citation(s) 24340035
Submission date May 30, 2013
Last update date Mar 22, 2019
Contact name Sandra Gehrke
E-mail(s) sandraandrea.gehrke@beiersdorf.com
Organization name Beiersdorf AG
Department Applied Skin Biology
Lab Special & Aged Skin
Street address Unnastra├če 48
City Hamburg
ZIP/Postal code 20245
Country Germany
 
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (12)
GSM1151333 10 AP_genomic DNA from subcutaneous adipose tissue
GSM1151334 10 GLM_genomic DNA from subcutaneous adipose tissue
GSM1151335 17 AP_genomic DNA from subcutaneous adipose tissue
This SubSeries is part of SuperSeries:
GSE47513 Gene expression and DNA methylation analysis of subcutaneous abdominal and gluteal adipose tissue
Relations
BioProject PRJNA205942

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47512_RAW.tar 183.1 Mb (http)(custom) TAR
GSE47512_non_normalized.txt.gz 50.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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