|
| Status |
Public on Oct 27, 2006 |
| Title |
HCaRG vs NEO |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Summary:
HEK293 cells were transfected with control plasmid (pcDNA1/Neo; Invitrogen) or with the plasmid encoding HCaRG by a standard calcium phosphate co-precipitation method. The clones expressing the highest levels of HCaRG, HCaRG clone 8 and 9 were used in this experiment, while clone transfected with vector alone, Neo clone, served as control.
Stable transfectants were synchronized and grown in the presence of 10% FBS for 48 h. Total RNAs were purified with the mini RNeasy kit (Qiagen).
Keywords: parallel sample
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| |
|
| Overall design |
Chips HG-U133 Plus 2.0:
- Labeling protocol: GeneChip IVT Labeling Kit.
- Hybridization: According to the manufacturer's protocol (Affymetrix).
- Scanner: GeneChip Scanner 3000.
- Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500.
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| |
|
| Contributor(s) |
El Hader C, Hamet P, Tremblay J |
| Citation(s) |
16033922 |
| Submission date |
Apr 28, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Johanne Tremblay |
| E-mail(s) |
johanne.tremblay@umontreal.ca
|
| Phone |
514-890-8000-12721
|
| Organization name |
CHUM Research Center, Hotel-Dieu
|
| Street address |
|
| City |
Montreal |
| State/province |
QUE |
| ZIP/Postal code |
H2W 1T8 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA95675 |
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