|
| Status |
Public on Jan 06, 2014 |
| Title |
CLK:BMAL1 is a pioneer-like transcription factor [MNase-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The mammalian circadian clock relies on the master genes CLOCK (CLK) and BMAL1 and drives rhythmic gene expression to regulate biological functions under circadian control. We recently uncovered a surprising disconnect between the rhythmic binding of CLK:BMAL1 on DNA and the transcription of its target genes, suggesting that they are regulated by as yet uncharacterized mechanisms. Here we show that rhythmic CLK:BMAL1 DNA binding promotes rhythmic chromatin opening. The underlying mechanisms include CLK:BMAL1 binding to nucleosomes and rhythmic chromatin modifications, including the incorporation of the histone variant H2A.Z. This rhythmic chromatin remodeling mediates the rhythmic binding of other transcription factors adjacent to CLK:BMAL1, suggesting that the activity and the tissue-specific expression of these other transcription factors contribute to the genome-wide CLK:BMAL1 heterogeneous transcriptional output. These data therefore indicate that the clock regulation of transcription relies on the rhythmic regulation of chromatin accessibility and suggest that the concept of pioneer function extends to acute gene regulation, well beyond the current confines of developmental/cell specification.
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| |
|
| Overall design |
Mouse liver nucleosome profile assayed by MNase-Seq over 6 time points of the 24h light:dark cycle (4 wild-type and 4 Bmal1-/- mice per time point). Illumina libraries containing a mononucleosome insert were sequenced using Ilumina HiSeq2000.
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| |
|
| Contributor(s) |
Menet JS, Pescatore S, Rosbash M |
| Citation(s) |
24395244, 29300726 |
| Submission date |
May 21, 2013 |
| Last update date |
Jul 09, 2019 |
| Contact name |
Jerome S Menet |
| E-mail(s) |
menet@bio.tamu.edu
|
| Organization name |
Texas A&M University
|
| Department |
Dept of Biology
|
| Street address |
301 Old Main Drive, Building 1530, ILSB
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (47)
|
|
| This SubSeries is part of SuperSeries: |
| GSE47145 |
CLK:BMAL1 is a pioneer-like transcription factor |
|
| Relations |
| BioProject |
PRJNA204308 |
| SRA |
SRP022946 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47142_MNase_allBM_ZT02_nucleosome.bw |
891.7 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allBM_ZT06_nucleosome.bw |
623.0 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allBM_ZT10_nucleosome.bw |
933.0 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allBM_ZT14_nucleosome.bw |
895.0 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allBM_ZT18_nucleosome.bw |
581.5 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allBM_ZT22_nucleosome.bw |
472.4 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allWT_ZT02_nucleosome.bw |
933.0 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allWT_ZT06_nucleosome.bw |
998.2 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allWT_ZT10_nucleosome.bw |
974.6 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allWT_ZT14_nucleosome.bw |
930.1 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allWT_ZT18_nucleosome.bw |
899.7 Mb |
(ftp)(http) |
BW |
| GSE47142_MNase_allWT_ZT22_nucleosome.bw |
891.3 Mb |
(ftp)(http) |
BW |
| GSE47142_Table_GEO_CB3217_GenomicLocation_AllPeaks.txt.gz |
65.7 Kb |
(ftp)(http) |
TXT |
| GSE47142_Table_GEO_MNase_signal_at_CB3217peaks_ALL.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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