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Series GSE47083 Query DataSets for GSE47083
Status Public on Jan 01, 2015
Title Scl specifies hemogenic endothelium and inhibits cardiogenesis via primed enhancers [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Scl/Tal1 confers hemogenic competence and prevents cardiomyogenesis in embryonic endothelium. Here we show that Scl both directly activates a broad gene regulatory network required for hematopoietic stem/progenitor cell (HS/PC) development, and represses transcriptional regulators required for cardiogenesis. Cardiac repression occurs during a short developmental window through Scl binding to distant cardiac enhancers that harbor H3K4me1 at this stage. Scl binding to hematopoietic regulators extends throughout HS/PC and erythroid development and spreads from distant enhancers to promoters. Surprisingly, Scl complex partners Gata 1 and 2 are dispensable for hematopoietic versus cardiac specification and Scl binding to the majority of its target genes. Nevertheless, Gata factors co-operate with Scl to activate selected transcription factors to facilitate HS/PC emergence from hemogenic endothelium. These results uncover a dual function for Scl in dictating hematopoietic versus cardiac fate choice and suggest a mechanism by which lineage-specific bHLH factors direct the divergence of competing fates.
Overall design Examination of Scl and Gata 1 & 2 target genes in ES cell derived day4.75 EB (embryoid body) Tie2+CD31+CD41- endothelial cells
Contributor(s) Org T, Duan D, Ferrari R, Montel-Hagen A, Van Handel B, Sasidharan R, Kerényi MA, Rubbi L, Fujiwara Y, Pellegrini M, Orkin SH, Kurdistani SK, Mikkola HK
Citation(s) 25564442
Submission date May 19, 2013
Last update date May 15, 2019
Contact name Tõnis Org
Phone 372-52-11484
Organization name University of Tartu
Department Institute of Molecular and Cell Biology
Lab Biotechnology
Street address Riia 23
City Tartu
ZIP/Postal code 51010
Country Estonia
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM1144557 WT_d475EB
GSM1144558 SclKO_d475EB
GSM1144559 Gata12KO_d475EB
This SubSeries is part of SuperSeries:
GSE47085 Scl specifies hemogenic endothelium and inhibits cardiogenesis via primed enhancers
BioProject PRJNA203512
SRA SRP022898

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47083_RAW.tar 12.9 Mb (http)(custom) TAR (of GTF)
GSE47083_WTvsSclKO_WTvsGata12KO.xlsx.gz 2.8 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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