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Series GSE46980 Query DataSets for GSE46980
Status Public on Dec 20, 2013
Title Quantitative single-cell RNA-seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: We applied cDNA molecule counting using unique molecular identifiers combined with high-throughput sequencing to study the transcriptome of individual mouse embryonic stem cells, with spike-in controls to monitor technical performance. We further examined transcriptional noise in the embryonic stem cells.
Overall design One 96-well plate of single-stranded cDNA libraries generated from 96 single R1 mouse embryonic stem cells sequenced on two lanes, and one 96-well plate of the same libraries further amplified by 9 PCR cycles sequenced on one lane.
Contributor(s) Islam S, Zeisel A, Lönnerberg P, Joost S, La Manno G, Zajac P, Linnarsson S
Citation(s) 24363023
Submission date May 15, 2013
Last update date May 15, 2019
Contact name Sten Linnarsson
Phone +46852487577
Organization name Karolinska Institutet
Department MBB
Lab Molecular Neurobiology
Street address Scheeles väg 2
City Stockholm
ZIP/Postal code 17177
Country Sweden
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (192)
GSM1280063 ESCell_1_ATTAGAC_single
GSM1280064 ESCell_2_ACGAGTC_single
GSM1280065 ESCell_3_TGACGTG_single
BioProject PRJNA203208
SRA SRP022764

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