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Series GSE46943 Query DataSets for GSE46943
Status Public on May 16, 2013
Title Transcription Factor Foxo1 Controls Memory CD8+ T Cell Responses To Infection [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Memory T cells provide immunity against pathogen reinvasion, but mechanisms of their long-term maintenance is unclear. Here we show that mice with the deletion of the transcription factor Foxo1 in activated CD8+ T cells had defective secondary but not primary responses to Listeria monocytogenes infection. Compared to short-lived effector T cells, memory precursor effector T cells expressed higher amounts of Foxo1 that promoted their generation and maintenance. Gene expression profiling and chromatin immunoprecipitation sequencing experiments revealed the chemokine receptor CCR7 and the transcription factor TCF1 as novel Foxo1-bound target genes with critical functions in memory T cell trafficking and transcriptional regulation. These findings demonstrate that Foxo1 is selectively incorporated into the genetic program that regulates memory but not effector CD8+ T cell responses to infection.
Overall design CD8+ T cells were isolated from wild-type or Foxo1tagBirA mice in which Foxo1 is endogenously biotinylated. Foxo1 binding targets in CD8+ cells were identified by using Foxo1 antibody- and Streptavidin- ChIP-Seq approaches.
Contributor(s) Kim MV, Ouyang W, Liao W, Zhang MQ, Li MO
Citation(s) 23932570
Submission date May 15, 2013
Last update date May 15, 2019
Contact name Willey Liao
Organization name New York Genome Center
Department Bioinformatics
Street address 101 Avenue of the Americas
City New York
State/province NY
ZIP/Postal code 10013
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (4)
GSM1141666 CD8+_Foxo1_Ab_ChIPseq
GSM1141667 CD8+_Foxo1_Biotin_ChIPseq
GSM1141668 CD8+_Input_Ab_ChIPseq
This SubSeries is part of SuperSeries:
GSE46944 Transcription Factor Foxo1 Controls Memory CD8+ T Cell Responses To Infection
BioProject PRJNA203120
SRA SRP022750

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Supplementary file Size Download File type/resource
GSE46943_RAW.tar 210.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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