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Series GSE46807 Query DataSets for GSE46807
Status Public on Jun 06, 2013
Title A TAF4 coactivator function for E proteins that involves enhanced TFIID binding
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The multisubunit TFIID plays a direct role in transcription initiation by binding to core promoter elements and directing preinitiation complex assembly. Although TFIID may also function as a coactivator through direct interactions with promoter-bound activators, mechanistic aspects of this poorly defined function remain unclear. Here biochemical studies show a direct TFIID-E protein interaction that (i) is mediated through interaction of a novel E protein activation domain (AD3) with the TAF homology (TAFH) domain of TAF4, (ii) is critical for activation of a natural target gene by an E protein and (iii) mechanistically, acts by enhancing TFIID binding to the core promoter. Complementary assays establish a gene-specific role for the TAFH domain in TFIID recruitment and gene activation in vivo. These results firmly establish TAF4 as a bona fide E protein coactivator, as well as a mechanism involving facilitated TFIID binding through direct interaction with an E protein activation domain.
Overall design Genome-wide profiling of mRNA levels in MEF lines with Taf4 loxp/- (ctrl), Taf4 -/- (ko), Taf4 -/- Tg:hTAF4 wt, and Taf4 -/- Tg: hTAF4 Δ (TAFH-deleted).
Contributor(s) Chen W, Zhang J, Geng H, Du Z, Nakadai T, Roeder RG
Citation(s) 23873942
Submission date May 09, 2013
Last update date May 15, 2019
Contact name Huimin Geng
Organization name UCSF
Department Department of Laboratory Medicine
Street address 513 Parnassus Ave., MSB S-1480
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1138582 Taf4 loxp/- (ctrl), replicate 1
GSM1138583 Taf4 loxp/- (ctrl), replicate 2
GSM1138584 Taf4 -/- (ko), replicate 1
BioProject PRJNA202371
SRA SRP022250

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Supplementary file Size Download File type/resource
GSE46807_GEO_values_TAF4_RNAseq_Tophat.Cufflinks_mm9_WeiYi.txt.gz 2.0 Mb (ftp)(http) TXT
GSE46807_RAW.tar 639.1 Mb (http)(custom) TAR (of BIGWIG)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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