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Series GSE46536 Query DataSets for GSE46536
Status Public on Jan 03, 2014
Title The Histone H3 Lysine 9 Methyltransferases G9a and GLP Regulate Polycomb Repressive Complex 2-Mediated Gene Silencing [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary G9a/GLP and Polycomb Repressive Complex 2 (PRC2) are two major epigenetic silencing machineries, which in particular methylate histone H3 on lysines 9 and 27 (H3K9 and H3K27), respectively. Although evidence of a crosstalk between H3K9 and H3K27 methylations has started to emerge, their actual interplay remains elusive. Here, we show that PRC2 and G9a/GLP interact physically and functionally. Moreover, combining different genome-wide approaches, we demonstrate that Ezh2 and G9a/GLP share an important number of common genomic targets, encoding developmental and neuronal regulators. Furthermore, we show that G9a enzymatic activity modulates PRC2 genomic recruitment to a subset of its target genes. Taken together, our findings demonstrate an unanticipated interplay between two main histone lysine methylation mechanisms, which cooperate to maintain silencing of a subset of developmental genes.
Overall design ChIP-seq has been performed for G9a and Ezh2 in wild type TT2 mES cells or in mES cells lacking both G9a and GLP (G9a-/-GLP-/-). As a control, input DNA was saved before immunoprecipitation. Note that the two inputs (Input_TT2_0 and Input_TT2_1) have been combined and used as control for Ezh2 and G9a ChIP-seq.
Contributor(s) Pontis J, Mozzetta C, Ait-Si-Ali S
Citation(s) 24389103
Submission date May 01, 2013
Last update date May 15, 2019
Contact name PONTIS Julien
Organization name UMR7216 CNRS
Street address 35, rue Hélène BRION
City Paris
ZIP/Postal code 75205
Country France
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1215218 Ezh2_TT2_1_ChIP-seq
GSM1215219 G9a_TT2_1_ChIP-seq
GSM1215220 G9a_WT_TT2_G9a-/-andGLP-/-_1_ChIP-seq
This SubSeries is part of SuperSeries:
GSE46545 The Histone H3 Lysine 9 Methyltransferases G9a and GLP Regulate Polycomb Repressive Complex 2-Mediated Gene Silencing
BioProject PRJNA200947
SRA SRP021942

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Supplementary file Size Download File type/resource
GSE46536_RAW.tar 2.3 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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