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Series GSE46439 Query DataSets for GSE46439
Status Public on Jul 08, 2013
Title Expression profiling of lung adenocarcinoma TPC tumor cells in Kras-driven NSCLC mouse model
Organism Mus musculus
Experiment type Expression profiling by array
Summary Sustained tumor progression has been attributed to a distinct population of tumor-propagating cells (TPCs). To identify TPCs relevant to lung cancer pathogenesis, we investigated functional heterogeneity in tumor cells isolated from Kras-driven mouse models of non-small cell lung cancer (NSCLC). CD24+ITGB4+Notchhi cells are capable of propagating tumor growth in both a clonogenic and an orthotopic serial transplantation assay. While all four Notch receptors mark TPCs, Notch3 plays a non-redundant role in tumor cell propagation in two mouse model and in human NSCLC. The TPC population is enriched after chemotherapy and the gene signature of mouse TPCs correlates with poor prognosis in human NSCLC. The unique role of Notch3 in tumor propagation may provide a therapeutic target for NSCLC
Overall design Primary lung adenocarcinoma tumor cells were FACS sorted based on expression of CD24, ITGB4 and Notch. TPC cells are defined by Cd24+ITGB4+ Notch(high), and the remainder tumor cells are non-TPC cells. Samples were derived from six mice.
Contributor(s) Zheng Y, Sweet-Cordero EA
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Submission date Apr 26, 2013
Last update date Jan 12, 2017
Contact name Yanyan Zheng
Organization name Stanford University
Street address 265 Campus Drive
City Stanford
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (11)
GSM1130158 TPC Cells_rep1
GSM1130159 TPC Cells_rep2
GSM1130160 TPC Cells_rep3
BioProject PRJNA200346

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Supplementary file Size Download File type/resource
GSE46439_RAW.tar 48.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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