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| Status |
Public on Jul 08, 2013 |
| Title |
Expression profiling of lung adenocarcinoma TPC tumor cells in Kras-driven NSCLC mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Sustained tumor progression has been attributed to a distinct population of tumor-propagating cells (TPCs). To identify TPCs relevant to lung cancer pathogenesis, we investigated functional heterogeneity in tumor cells isolated from Kras-driven mouse models of non-small cell lung cancer (NSCLC). CD24+ITGB4+Notchhi cells are capable of propagating tumor growth in both a clonogenic and an orthotopic serial transplantation assay. While all four Notch receptors mark TPCs, Notch3 plays a non-redundant role in tumor cell propagation in two mouse model and in human NSCLC. The TPC population is enriched after chemotherapy and the gene signature of mouse TPCs correlates with poor prognosis in human NSCLC. The unique role of Notch3 in tumor propagation may provide a therapeutic target for NSCLC
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| Overall design |
Primary lung adenocarcinoma tumor cells were FACS sorted based on expression of CD24, ITGB4 and Notch. TPC cells are defined by Cd24+ITGB4+ Notch(high), and the remainder tumor cells are non-TPC cells. Samples were derived from six mice.
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| Contributor(s) |
Zheng Y, Sweet-Cordero EA |
| Citation missing |
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| |
| Submission date |
Apr 26, 2013 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Yanyan Zheng |
| E-mail(s) |
yanyanzh@stanford.edu
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| Organization name |
Stanford University
|
| Street address |
265 Campus Drive
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| City |
Stanford |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA200346 |
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