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| Status |
Public on Apr 01, 2014 |
| Title |
The transcriptomic response to finasteride in the Silurana tropicalis testis |
| Organism |
Xenopus tropicalis |
| Experiment type |
Expression profiling by array
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| Summary |
An ex vivo protocol was optimized to expose testis tissues from juvenile Silurana tropicalis to 5 uM finasteride. Testis tissues were incubated in L-15 media with (n = 8) and without (n = 8) finasteride for 6 h at 26 °C on top of an orbital shaker. Afterwards, the tissues were flash frozen on dry ice and RNA was isolated using Trizol. The isolated RNA of four control tissue samples and four finasteride exposed tissue samples were then analyzed using a custom microarray for S. tropicalis containing 44,000 probes .
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| Overall design |
Testis tissues were incubated in L-15 media with (n = 8) and without (n = 8) finasteride for 6 h at 26 °C
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| Contributor(s) |
Valerie L, Sonja B, Chris M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 24, 2013 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Christopher Joseph Martyniuk |
| E-mail(s) |
CMARTYN@UFL.EDU
|
| Organization name |
UF
|
| Department |
Physiological Sciences
|
| Street address |
CEHT Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
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| Platforms (1) |
| GPL15626 |
Agilent-035843 Genotypic_EA519_Xenopus_tropicalis_GE_4x44k |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA198905 |
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