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Series GSE46340 Query DataSets for GSE46340
Status Public on Oct 17, 2013
Title RNA-seq reveals changes in sphingolipid metabolism upon transformation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We sequenced mRNA from three independent pairs of mock and c-SRC transformed mouse embryonic fibroblasts and examined changes in expression of sphingolipid metabolism genes
Overall design Three independent cultures of NIH3T3 MEFs were retrovirally transduced with either pBabe-Puro, og pBabe-Puro containing c-srcY527F. After a 7 day of selection in puromycine the three vector and three src transduced pools were propagated for RNA sequencing.
Contributor(s) Rohde M, Jäättelä M
Citation(s) 24029234
Submission date Apr 24, 2013
Last update date May 15, 2019
Contact name Mikkel Rohde
Phone +45 35 25 73 38
Organization name Danish Cancer Society
Lab Cell Death and Metabolism
Street address Strandboulevarden 49
City Copenhagen
ZIP/Postal code 2100
Country Denmark
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM1128788 NIH3T3 pBabe-puro-1
GSM1128789 NIH3T3 pBabe-puro-2
GSM1128790 NIH3T3 pBabe-puro-3
BioProject PRJNA198770
SRA SRP021484

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Supplementary file Size Download File type/resource
GSE46340_Babe_vs_Src.txt.gz 3.7 Mb (ftp)(http) TXT
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