|
| Status |
Public on Oct 17, 2013 |
| Title |
RNA-seq reveals changes in sphingolipid metabolism upon transformation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced mRNA from three independent pairs of mock and c-SRC transformed mouse embryonic fibroblasts and examined changes in expression of sphingolipid metabolism genes
|
| |
|
| Overall design |
Three independent cultures of NIH3T3 MEFs were retrovirally transduced with either pBabe-Puro, og pBabe-Puro containing c-srcY527F. After a 7 day of selection in puromycine the three vector and three src transduced pools were propagated for RNA sequencing.
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| |
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| Contributor(s) |
Rohde M, Jäättelä M |
| Citation(s) |
24029234 |
| Submission date |
Apr 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Mikkel Rohde |
| E-mail(s) |
mr@cancer.dk
|
| Phone |
+45 35 25 73 38
|
| Organization name |
Danish Cancer Society
|
| Lab |
Cell Death and Metabolism
|
| Street address |
Strandboulevarden 49
|
| City |
Copenhagen |
| ZIP/Postal code |
2100 |
| Country |
Denmark |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA198770 |
| SRA |
SRP021484 |
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