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Series GSE46197 Query DataSets for GSE46197
Status Public on Jul 15, 2014
Title Expression data from miR-132 overexpressing immortalized human mammary fibroblasts and from mammary epithelial columnar cell hyperplasia cells co-cultured with miR-132 overexpressing fibroblasts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Columnar cell hyperplasia (CCH) is the first histologically identifiable lesion in the breast with premalignant potential. Altered miRNA expression in the stroma surrounding CCH compared to normal tissue was discovered. The effect of upregulation of one specific miRNA was investigated by gene expression array in human mammary fibroblasts as well as in epithelial CCH cells coculterd with miR-132 oversexpressing human mammary fibroblasts.
We used microarrays to detail the effects of miR-132 in human mammary fibroblasts and identified multiple altered genes and gene pathways both in the fibroblasts and in cocultured human mammary epithelial CCH cells.
 
Overall design Immortalized human mammary fibroblasts were transfected in duplicates with miRNA mimics for miR-132 or negative control and RNA was extracted 24 h after transfection. Human mammary epithelial columnar cell hyperplasia cells were cocultured with immortalized human mammary fibroblasts transfected with either miR-132 mimic or negative control 24 hours prior to RNA extraction. The RNA from all six samples was hybridized on Affymetrix microarrays.
 
Contributor(s) Landberg G, Nilsson S
Citation(s) 25122196
Submission date Apr 18, 2013
Last update date Jan 25, 2019
Contact name Sofie Björner
Organization name Lund University
Street address Jan Waldenströms gata 59
City Malmö
ZIP/Postal code 20502
Country Sweden
 
Platforms (1)
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (6)
GSM1126120 Fibroblasts control mimic rep 1
GSM1126121 Fibroblasts control mimic rep 2
GSM1126122 Fibroblasts miR-132 mimic rep 1
This SubSeries is part of SuperSeries:
GSE46199 Columnar cell hyperplasia
Relations
BioProject PRJNA198022

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46197_RAW.tar 24.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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