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| Status |
Public on Apr 29, 2013 |
| Title |
High Resolution Mapping of H1 Linker Histone Variants in Embryonic Stem Cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
H1 linker histones facilitate higher order chromatin folding and are essential for mammalian development. To achieve high resolution mapping of H1 variants H1d and H1c in embryonic stem cells (ESCs), we have established a knock-in system and shown that the N-terminally tagged H1 proteins are functionally interchangeable to their endogenous counterparts in vivo. H1d and H1c are depleted from GC- and gene-rich regions and active promoters, inversely correlated with H3K4me3, but positively correlated with H3K9me3 and associated with characteristic sequence features. Surprisingly, both H1d and H1c are significantly enriched at major satellites, which display increased nucleosome spacing compared with bulk chromatin. While also depleted at active promoters and enriched at major satellites, overexpressed H10 displays differential binding patterns in specific repetitive sequences compared with H1d and H1c. Depletion of H1c, H1d and H1e causes pericentric chromocenter clustering and de-repression of major satellites. These results integrate the localization of an understudied type of chromatin proteins, namely the H1 variants, into the epigenome map of mouse ESCs, and we identify significant changes at pericentric heterochromatin upon depletion of this epigenetic mark.
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| Overall design |
Mapping linker histone H1 variants H1d, H1c and H10 as well as 3 core histone modifications in mouse ESC genome.
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| Contributor(s) |
Cao K, Lailler N, Dong X, Bouhassira EE, Fan Y |
| Citation(s) |
23633960 |
| Submission date |
Apr 17, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuhong Fan |
| Organization name |
Georgia Institute of Technology
|
| Department |
School of Biology and the Petit Institute for Bioengineering and Bioscience
|
| Street address |
IBB 2313, 315 Ferst Drive
|
| City |
Atlanta |
| State/province |
Georgia |
| ZIP/Postal code |
30332 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA197331 |
| SRA |
SRP021132 |
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