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Series GSE46114 Query DataSets for GSE46114
Status Public on Apr 02, 2014
Title Mapping of Notch1 binding sites in a mouse immature T cell line
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We used an immature mouse T cell line engineered to express a biotinylated form of the cleaved form of Notch1 (ICN1). ICN1-bound sites were precipitated with streptavidin-coated beads and subjected to ChIP-sequencing.
Overall design Beko cells correspond to a spontaneous T lymphoma immature cell line derived from a TCRb deficient mouse. These cells were engineered to express a biotin-tagged-ICN1 and the bacterial biotin ligase BirA (Bio-ICN1) or just BirA as a control (Bio). Chromatin from both cell lines was subjected to strepatavidin-mediated precipitation and subjected to sequencing with the Illumina GAII sequencer as single end 36 base pair reads.
Contributor(s) Kastner P
Citation(s) 24643801
Submission date Apr 16, 2013
Last update date May 15, 2019
Contact name Susan Chan
Phone 33 3 88 65 34 72
Organization name IGBMC
Street address 1, rue Laurent Fries
City Illkirch
ZIP/Postal code 67404
Country France
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM1123915 control streptavidin ChIP-seq of BirA expressing Beko cells
GSM1123916 Strepatvidin ChIP-seq of Beko cells expressing Biotinylated ICN1
BioProject PRJNA197294
SRA SRP021157

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Supplementary file Size Download File type/resource
GSE46114_RAW.tar 344.1 Mb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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