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| Status |
Public on Apr 02, 2014 |
| Title |
Mapping of Notch1 binding sites in a mouse immature T cell line |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We used an immature mouse T cell line engineered to express a biotinylated form of the cleaved form of Notch1 (ICN1). ICN1-bound sites were precipitated with streptavidin-coated beads and subjected to ChIP-sequencing.
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| Overall design |
Beko cells correspond to a spontaneous T lymphoma immature cell line derived from a TCRb deficient mouse. These cells were engineered to express a biotin-tagged-ICN1 and the bacterial biotin ligase BirA (Bio-ICN1) or just BirA as a control (Bio). Chromatin from both cell lines was subjected to strepatavidin-mediated precipitation and subjected to sequencing with the Illumina GAII sequencer as single end 36 base pair reads.
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| Contributor(s) |
Kastner P |
| Citation(s) |
24643801 |
| Submission date |
Apr 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Susan Chan |
| E-mail(s) |
scpk@igbmc.fr
|
| Phone |
33 3 88 65 34 72
|
| Organization name |
IGBMC
|
| Street address |
1, rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67404 |
| Country |
France |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
| GSM1123915 |
control streptavidin ChIP-seq of BirA expressing Beko cells |
| GSM1123916 |
Strepatvidin ChIP-seq of Beko cells expressing Biotinylated ICN1 |
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| Relations |
| BioProject |
PRJNA197294 |
| SRA |
SRP021157 |
