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Series GSE46066 Query DataSets for GSE46066
Status Public on Oct 10, 2013
Title Gene regulation and priming by Topoisomerase IIα in embryonic stem cells [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Topoisomerases are essential for resolving topological problems in the genome, while their function in gene regulation, especially during cellular differentiation, remains unknown. We reveal that the expression of two Topo II isoforms, Top2a and Top2ß, is characteristic of dividing and postmitotic tissues, respectively. In embryonic stem cells, Top2a preferentially binds to promoters embedded in an active chromatin environment. Inhibition of Top2a activity results in misregulation of target gene expression that accompanies accumulation of double-strand breaks. Common targets of Top2a and Top2ß are housekeeping genes while their unique targets are involved in proliferation/pluripotency and neurogenesis, respectively. Moreover, a subset of Top2a targets exhibit bivalent chromatin state that is resolved upon differentiation concomitant with their activation and occupancy by Top2ß, a feature further observed for long genes. These findings suggest that Top2a not only contributes to stem cell transcriptome regulation but may also prime developmental genes for subsequent activation upon differentiation.
Overall design mRNA profiles of DMSO and ICRF-193 treated mESCs were generated by deep sequencing in triplicates. ICRF-193 is a well established catalytic inhibitor of Topoisomerase II, hence, we used ICRF-193 to the elucidate role of Top2a catalytic activity on transcription by genome wide transcription profiling.
Contributor(s) Thakurela S, Garding A, Jung J, Burger L, Tiwari V
Citation(s) 24072229
Submission date Apr 15, 2013
Last update date May 15, 2019
Contact name Sudhir Thakurela
Organization name HSCRB
Street address 7 Divinity Avenue
City Cambridge
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1122754 DMSO reps1-3
GSM1122755 ICRF reps1-3
This SubSeries is part of SuperSeries:
GSE46070 Gene regulation and priming by Topoisomerase IIα in embryonic stem cells
BioProject PRJNA197150
SRA SRP021089

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Supplementary file Size Download File type/resource
GSE46066_ICRFVsDMSO.txt.gz 1.1 Mb (ftp)(http) TXT
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