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Series GSE45983 Query DataSets for GSE45983
Status Public on May 20, 2014
Title Characterization and parental contribution of piRNAs and endo-siRNAs to mouse zygotes.
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Transposable elements (TEs) are widely represented in eukaryotic genomes. Recently, a set of small RNAs known as rasRNAs (repeat-associated small RNAs) have been related to the down-regulation of TEs conferring a means to safeguard genome integrity. Two key members of the rasRNAs group are piRNAs and endo-siRNAs. In this study, we have performed a comparative analysis of piRNAs and endo-siRNAs present in mouse oocytes, spermatozoa and zygotes, identified by deep sequencing and bioinformatic analysis. Both piRNAs and endo-siRNAs regulate TEs in addition to other repetitive elements such as tRNAs and rRNAs, suggesting an alternative role of rasRNAs with regard to translation regulation. The detection of piRNAs and endo-siRNAs in sperm cells and revealed also in zygotes, hints to their potential delivery to oocytes during fertilization. However, a comparative assessment of the three cell types indicates that both piRNAs and endo-siRNAs are mainly maternally inherited. Finally, we have assessed the role of the different rasRNA molecules in connection with amplification processes by way of the “ping-pong cycle”. Our results suggest that the ping-pong cycle can act on other rasRNAs, such as tRNA- and rRNA-derived fragments, thus not only being restricted to TEs during gametogenesis, as was evidenced in spermatozoa, oocytes and zygotes.
Overall design Comparative analysis from deep sequencing of piRNAs and endo-siRNAs in mouse oocytes, spermatozoa and zygotes
Contributor(s) Garcia-Lopez J, Hourcade J, Alonso L, Cárdenas DB, del Mazo J
Citation(s) 24769224
Submission date Apr 11, 2013
Last update date May 15, 2019
Contact name Jesus Garcia Lopez
Phone +34 918373112
Organization name Cib (CSIC)
Department Cellular and Molecular Biology
Lab Molecular biology of gametogenesis
Street address C/Ramiro de Maeztu 9
City Madrid
ZIP/Postal code 28040
Country Spain
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM1121087 Oocytes
GSM1121088 Spermatozoa
GSM1121089 Zygotes
BioProject PRJNA197386
SRA SRP021143

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Supplementary file Size Download File type/resource
GSE45983_RAW.tar 176.4 Mb (http)(custom) TAR (of CSV, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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