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Series GSE45958 Query DataSets for GSE45958
Status Public on Jul 01, 2013
Title Radiation-induced epigenetic DNA methylation modification of radiation-response pathways
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary Treatment of tumors with ionizing radiation for cancer therapy induces biological responses that include changes in cell cycle, activation of DNA repair mechanisms, and induction of apoptosis or senescence programs. What is not known is whether ionizing radiation induces an epigenetic DNA methylation response or whether epigenetic changes occur in genes in pathways classically associated with the radiation response. We exposed breast cancer cells to 0, 2, or 6 Gy and determined global DNA methylation at 1, 2, 4, 8, 24, 48, and 72 hours post-irradiation. We found that radiation treatment resulted in a DNA methylation response and that cell cycle, DNA repair, and apoptosis pathways were enriched in genes are were differentially-methylated.
Overall design DNA methylation profiling of ionizing radiation treated cells using the Infinium HumanMethylation450 BeadChip.
Contributor(s) Antwih DA, Gabbara KM, Lancaster WD, Ruden DM, Zielske SP
Citation(s) 23880508
Submission date Apr 10, 2013
Last update date Mar 22, 2019
Contact name Steven P Zielske
Organization name Wayne State University
Department Department of Radiation Oncology
Street address 540 E. Canfield
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (24)
GSM1120231 0 Gy, 0 hr (1)
GSM1120232 0 Gy, 0 hr (2)
GSM1120233 0 Gy, 0 hr (3)
BioProject PRJNA196941

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45958_RAW.tar 183.1 Mb (http)(custom) TAR
GSE45958_unmethylated_and_methylated_signal.txt.gz 99.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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