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Series GSE45911 Query DataSets for GSE45911
Status Public on Dec 31, 2013
Title Methylome of CD4+ T cell subsets
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary We compared the methylated and non-methylated regions in the genome of ex vivo-isolated naive CD4+ T cells, Th1 cells, Th17 cells and regulatory T cells by methyl-CpG binding domain protein sequencing (MBD-seq). Naive T cells and Th1 cells share more methylated regions than naive T cells and Th17 cells or Th1 and Th17 cells. However, analysis of the non-methylated regions revealed the highest similarity between Th1 and Th17 cells. Another aim was the analysis of the Th17 lineage on the basis of the methylome. We searched for regions absent in the methylome of Th17 but present in naive T cells, Th1 cells and regulatory T cells. Here, we identified differential methylation in the loci of Il17a, Chn2, Dpp4 and Dclk1.
Overall design CD4+ T effector cells were prepared ex vivo, stimulated with PMA/Ionomycin, subjected to a comercially available cytokine secretion kit (IL-17A and IFNg), stained by adding fluorescence-labeled antibodies against CD3, CD4 and CD45RB and sorted by flow cytometry. We sorted naive CD4+ T cells (CD3+CD4+CD45RB_high), Th1 cells (CD3+CD4+CD45RB_low_IFNg+IL17A-), Th17 cells (CD3+CD4+CD45RB_low_IFNg-IL17A+) and regulatory T cells (CD3+CD4+CD25++).
Contributor(s) Floess S, Yang B
Citation(s) 25593324
Submission date Apr 09, 2013
Last update date May 15, 2019
Contact name Stefan Floess
Organization name Helmholtz-Centre for Infection Research
Department Experimental Immunology
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM1119584 MBD-Tnaive
GSM1119585 MBD-Th1
GSM1119586 MBD-Th17
BioProject PRJNA196635
SRA SRP020662

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Supplementary file Size Download File type/resource
GSE45911_Methylation_T_cell_subsets.xls.gz 779.7 Kb (ftp)(http) XLS
GSE45911_Th17_vs_other_T_cell_subsets.txt.gz 441.8 Kb (ftp)(http) TXT
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