|
| Status |
Public on Sep 16, 2013 |
| Title |
Cytokine-induced changes in the gene expression profile of human cerebral microvascular endothelial cell-line, hCMEC/D3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In the current study, we aimed to investigate the gene expression pattern in hCMEC/D3 cells using mRNA microarray analysis and to establish wheather hCMEC/D3 cells are a suitable in vitro human BBB model of neuroinflammation.
|
| |
|
| Overall design |
To identify the major molecular process involved in cytokine-induced changes in brain endothelial phenotypic characteristics and subsequently function, we analysed changes in mRNA expression profile after activation of hCMEC/D3 cells by TNFa and IFNg and results were contrasted to unstimulated cells. hCMEC/D3 cells were grown on collagen-coated six-well plates and treated with 10 ng/ml of TNFa and IFNg (R&D systems, Abingdon, Oxon, UK) for 24 h
|
| |
|
| Contributor(s) |
Lopez-Ramirez MA, Sharrack B, Male DK, Romero IA |
| Citation(s) |
24050303 |
| Submission date |
Apr 08, 2013 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Miguel Alejandro Lopez-Ramirez |
| E-mail(s) |
malopezramirez@ucsd.edu
|
| Phone |
858-822-6487
|
| Organization name |
University of California, San Diego
|
| Department |
School of Medicine
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
California/San Diego |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA196534 |
Less...
More...